| Malaria ELISA | rnThe Malaria ELISA is intended for the qualitative determination of antibodies against Plasmodium in human serum or plasma (citrate). | rn
|---|---|
| Principal of the Assay | rnThe qualitative immunoenzymatic determination of antibodies against Plasmodium is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells re pre-coated with Plasmodium antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material hoseradish peroxidase (HRP) labeled anti-human IgG and IgM conjugate are added. This conjugate binds to the capture Plasmodium-specific antibodies.The immune complecx formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of Plasmodium-specific antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an ELISA microwell plate reader. | rn
| Recombinant Antigen | rnRecombinant CSP and MSP1 proteins from Plasmodium vivaxandPlasmodium falciparum. | rn
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