Type IV collagen is a major structural component of basement membranes. It is a multimeric protein composed of 3 alpha subunits， which are encoded by 6 different genes， alpha 1 through alpha 6. Each of these alpha subunits can form a triple helix structure with 2 other subunits to form type IV collagen. The Goodpasture syndrome is a condition in which autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are restricted basically to the non-collagenous C-terminal domain of the protein. There are numerous alternative transcripts that appear to be unique to the human COL4A3 and alternative splicing is limited to the six exons that encode this C-terminal domain. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to the Alport syndrome are also situated within the exons that encode this C-terminal region. COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Several exons of COL4A3 are interspersed with exons of an uncharacterized gene which is on the opposite strand.
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昆虫Sf9细胞中重组表达
溶解建议
Sterile Filtered clear solution. The protein solution （0.6 mg/ml） contains 20mM Hepes， pH 8.0， and 4M Urea
保存建议
Recommendations for storage buffer: ion strength 100mM， neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent. Storage temperature is -70° to -80° C. Please prevent freeze-thaw cycles.
