The Arginine-Vasopressin （Arg-Vasopressin） BioAssay ELISA Kit （Rat） is a quantitative competitive assay for the detection of Arg-Vasopressin in rat serum，plasma and other biological fluids.Detection Range：0.313-20ng/mlSensitivity：<0.188ng/mlPrecision： Intra-Assay CV： <8%Inter-Assay CV： <10%Assay Principle：The microtiter plate provided in this kit has been pre-coated with Arg-Vasopressin. Arg-Vasopressin in the standard or sample competes with a fixed amount of plate-coated Arg-Vasopressin for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate，after which Streptavidin-HRP （SABC） is added to each well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of Arg-Vasopressin in the samples is determined by comparing the absorbance of the samples to the standard curve.Kit Components：*369765A： Microtiter Strips，8x12 wells.*369765B： Standard，2 vials369765C： Sample/Standard Dilution Buffer，1x20ml369765D： Antibody-Biotin Concentrate，1x160ul369765E： Antibody Dilution Buffer，1x10ml369765F： Streptavidin-HRP Conjugate （SABC），1x120ul369765G： SABC Dilution Buffer，1x10ml369765H： TMB Substrate，1x10ml369765J： Stop Solution，1x10ml369765K： Wash Buffer （25X），1x30mlStorage and Stability：Store unopened *369765A at 4ºC; store at -20ºC once opened. Store unopened *369765B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Assay Summary：1. Wash plate 2 times before adding standards，samples and control （zero） to wells.2. Add 50ul standard or sample to each well.3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
