Hoechst 33342 is a cell-permeable fluorescent DNA stain. It binds minor groove of AT-rich regions and is used to quantify DNA in viable cells.Synonyms:2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole; 2-[2-(4-Ethoxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole; BXI 72; Bisbenzimide; HOE 33342; Ho 342; Hoechst H 33342; NSC 334072; 2’-(p-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bibenzimidazoleCAS No:23491-52-3Molecular Formula:C₂₇H₂₈N₆O·3HCl·x7H2OMolecular Weight:688.04Appearance:Supplied as a yellow solidPurity (HPLC):≥98% (Typically ≥99%)Solubility:Soluble to 100mM in water and to 50mM in DMSO. Some solutions can be difficult to obtain and can be encouraged by rapid stirring, sonication or gentle warming (in a 45-60°C water bath).Method for Determining Identity: Proton NMR Spectroscopic and Mass Spectrometric analysisMicroanalysis:Theoretical: %C: 47.13 %H: 6.59 %N: 12.21Absorption:λmax = 352nmStorage and Stability:May be stored at -20°C. Stable for 6 months after receipt at -20°C. Light sensitive. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
