Methicillin sodium is a narrow spectrum B-lactam antibiotic related to Penicillin. B-lactams interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to B-lactams is commonly due to cells containing plasmid encoded B-lactamases. Methicillin is mostly resistant to B-lactamases. Methicillin targets primarily the cell wall of Gram positive organisms especially Staphylococcus aureus.Water (Karl Fisher):≤10%Melting Point:196197°COptical Rotation:+225°Solubility:Water: ~0.3mg/mlAcetone: 0.35mg/mlEthanol: 40mg/mlMIC:Staphylococcus aureus: 0.5ug/ml-32ug/mlStorage:-20°C
