- 中文名称
Peroxide Assay Kit, BioAssay, Colorimetric
- 英文名字
- Peroxide Assay Kit, BioAssay, Colorimetric
- 供应商
- USBiological
- 产品货号
- P3351-71
- 产品报价
- ￥1/250Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory arthritis and neurodegenerative diseases.Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. Peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.Key Features:Sensitive and accurate. Enhanced color intensity using sorbitol.Detection range 0.2uM (7 ng/mL) to 30uM (1,020 ng/mL) H2O2 in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Applications:Direct Assays: H2O2 in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium).Pharmacology: effects of drugs on peroxide metabolism.Kit Contents: (250 tests in 96-well plates)Reagent A: 1mlReagent B: 50mlStandard: 100ul 3% stabilized H2O2.Kit shipped at room temperature.Storage conditions. The kit is shipped at room temperature. Store all reagents at 4 °C. Shelf life: 6 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Sample Treatment: several chemicals are known to interfere and should be avoided in sample preparation. These include ascorbic acid, EDTA, heparin, DMSO (>0.02%), NP-40 (>0.6%), SDS (>0.12%), Tris (>8mM) and ethanol (>0.4%). Samples can be analyzed immediately after collection, or stored in aliquots at –20 °C. Avoid repeated freeze-thaw cycles.Reagent Preparation: Equilibrate to room temperature before assay. Prepare enough Detection Reagent by mixing 1 volume of Reagent A with 100 volumes Reagent B.Procedure using 96-well plate:1. Standards. Prepare fresh standards on the day of assay. Pipette 5ul 3% H2O2 and mix well with 495ul H2O in a 1.5-mL Eppendorf tube. Mix 5ul of this solution with 1465ul H2O. The final H2O2 concentration is 30uM (labeled “Premix”). Dilute standard as shown in the Table.2. Transfer 40ul diluted standards and each sample into separate wells of a clear flat-bottom 96-well plate. Add 200ul Detection Reagent to all standards and samples.No Premix+H2O Vol (ul) H2O2 (uM)1 100ul+0ul 100 302 80ul+20ul 100 243 60ul+40ul 100 184 40ul+60ul 100 125 30ul+70ul 100 96 20ul+80ul 100 67 10ul+90ul 100 38 0ul+100ul 100 03. Incubate 30 min at room temperature and read optical density at 540-610nm (peak absorbance at 585nm).Note: if in rare cases, precipitation occurs after adding the Detection Reagent to a sample, transfer the whole reaction mixture of this sample well into a 1.5-mL Eppendorf tube and centrifuge 2 min at 14,000 rpm. Carefully remove 200ul supernatant into a clean well and read OD. Multiply the OD reading by 1.2 to account for the volume change.Calculation:Subtract blank OD (water, #8) from the standard OD values and plot the OD against H2O2 concentrations. Subtract blank OD from Sample OD. Determine the sample peroxide content from the standard curve.Conversions: 1uM H2O2 equals 34 ng/mL or 34 ppb.Materials Required, But Not Provided:Pipetting devices and accessories, 96-well plates and plate reader.Examples:Duplicate assays for goat serum, human serum, 293 cell culture medium and fresh human urine gave peroxide content of 8.7± 2.8, 14.2 ± 2.7, 2.4 ± 0.0 and 1.4 ± 0.6uM (n=2).
- 产品特点
- 针对Peroxide Assay Kit, BioAssay, Colorimetric该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Peroxide Assay Kit, BioAssay, Colorimetric产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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