Protein A is 56 kDa protein molecule found in the cell wall of the bacterium Staphylococcus aureus. Protein A binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region. Protein A does not bind to all IgG's equivalently and binding varies among species. Even within a species, Protein A interacts with some subclasses of IgG and not others. Protein A is well suited for purification of most rabbit, mouse, rat, and certain human IgG class of immunoglobulin. Rockland offers Protein G or recombinant Protein A/G when recommended by Rockland for optimal purification.
产品描述
Protein A purification is a process for isolating the IgG type of immunoglobulin protein from a complex mixture. The starting material is usually but not limited to polyclonal antisera, tissue culture supernatant, or ascites fluid. Typically protein A purification is performed when IgG enrichment is desired, and is often used to purify IgG prior to conjugation to a reporter molecule. The IgG is bound to an immobilized Protein A matrix, non-IgG impurities are washed away, lastly the IgG fraction is eluted and recovered. Rockland's Protein A purification process is ideal to recover a highly active IgG fraction from complex starting material.
产品形式
该PROTEIN A PURIFICATION (up to 100 ml serum or 100 ml of ascites or 500 ml of processed and concentrated tissue culture supernate) - CUST22M的详细信息查看Rockland提供的产品说明书
保存建议
该PROTEIN A PURIFICATION (up to 100 ml serum or 100 ml of ascites or 500 ml of processed and concentrated tissue culture supernate) - CUST22M的详细信息查看Rockland提供的产品说明书
