SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups). SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343nm, the reaction can be easily followed. Contains a disulfide linkage in its spacer arm with a length of 6.8.Applications: • Reversible crosslinking of proteins • N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains • Useful for adding sulfhydryl groups to proteins by attachment to lysine residues • Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugatesSolubility:DSMO or DMFStorage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
