Members of the Argonaute (Ago) protein family are internal to RISC function. Argonaute proteins bind to mature microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Argonautes are needed for miRNA-induced silencing and contain two conserved RNA binding domains: a PAZ domain that can bind the single stranded 3’ end of the mature miRNA and a PIWI domain that structurally resembles ribonuclease-H and functions to interact with the 5’ end of the guide strand. Some argonautes, for example human Ago2, cleave target transcripts directly; argonautes may also recruit additional proteins to achieve translational repression. The human genome encodes eight Argonaute proteins divided by sequence similarities into two families: AGO (with four members present in all mammalian cells and called E1F2C/hAgo in humans), and PIWI (found in the germ line and hematopoietic stem cells). Anti-Ago Antibody is ideal for research in Alternative Splicing, Gene Expression and Gene Silencing.
Anti-Ago Antibody was produced in mice by repeated immunization with human Ago2 protein (fusion protein containing amino acids 47-859 of human Ago2).
来源宿主
Mouse
反应性
H. sapiens (Human); Mus musculus (Mouse); Other (wide range)
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
