alpha-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes alpha-Mannosidase and leads to deterioration of the central nervous system in children. Nonradioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405nm after addition of the stop reagent is directly proportional to the enzyme activity.Intended Use:Quantitative determination of alpha-Mannosidase activity using a p-nitrophenol based substrate at 405nmApplications:alpha-Mannosidase activity determination in biological samples (e.g. plasma, serum, tissue and culture media.) Range:1-250U/L for a 10 minute reaction.Detection limit: 1U/LKit Components:Substrate, 1x10mlStop Reagent, 1x12mlStandard, 1x1ml 12.5mM Nitrophenol Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
