Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. B-galactosidase (b-gal; b-gal) is an enzyme that hydrolyzes b-galactosides (such as lactose and various glycoproteins) into simple sugars. B-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. B-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified.Source:B-Galactosidase, expressed in E.coli.Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.Recommended Dilution:ELISA: 1:8000-1:32,000Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at 4°C. Do Not Freeze Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
