iFluor488-dUTP：更亮的信号，更强的光稳定性

染料修饰的脱氧尿苷5'-三磷酸盐是通过常规酶掺入方法（如逆转录、缺口翻译、随机引物标记或PCR）生产染料标记DNA的最常见方法之一。这种酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。这种iFluor 488 dUTP缀合物使用SpectrumGreen?滤光片组作为绿色荧光色（SpectrumGreen?是Vysis的商标）。与常用的绿色探针（如荧光素-12-dUTP）相比，iFluor 488提供了更亮的信号，具有更强的光稳定性，不受pH值的影响。

艾美捷iFluor488-dUTP：

货号：AAT-17044

单位大小：25 纳摩尔

分子量：1152.83

溶剂：水

校正因子（260 纳米）：0.21

校正因子（280 纳米）：0.11

消光系数（cm -1 M -1）：75000

激发（纳米）：491

发射（纳米）：516

量子产率：0.9

H-短语：H303, H313, H333

危险符号：XN

预期用途：仅限研究使用（RUO）

R-短语：R20, R21, R22

储存：冷冻（< -15 °C）；尽量减少光照暴露

图：荧光素和iFluor?488 dUTP标记的端粒探针在中期HeLa细胞中的荧光原位杂交。

iFluor488-dUTP文献参考：

Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.

Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro

Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77

Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.

Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe

Journal: TheScientificWorldJournal (2012): 609597

SNP genotyping using microsphere-linked PNA and flow cytometric detection.

Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and K?lvraa, Steen and Nielsen, Kirsten Vang and Nex?, Bj?rn A

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6

[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].

Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai

Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90

Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.

Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong

Journal: Biotechnology and bioengineering (2003): 127-33