染料修饰的脱氧尿苷5'-三磷酸盐是通过常规酶掺入方法(如逆转录、缺口翻译、随机引物标记或PCR)生产染料标记DNA的最常见方法之一。这种酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。这种iFluor 488 dUTP缀合物使用SpectrumGreen?滤光片组作为绿色荧光色(SpectrumGreen?是Vysis的商标)。与常用的绿色探针(如荧光素-12-dUTP)相比,iFluor 488提供了更亮的信号,具有更强的光稳定性,不受pH值的影响。
艾美捷iFluor488-dUTP:
货号 AAT-17039
单位大小 100纳摩尔、25纳摩尔、100纳摩尔
分子量 1152.83
溶剂 水
危害语句代码 H303, H313, H333
危害标识符 XN
预期用途 仅供研究使用(RUO)
风险语句代码 R20, R21, R22
储存条件 冷冻(<-15°C);尽量减少光照
图:荧光素和iFluor?488 dUTP标记的端粒探针在中期HeLa细胞中的荧光原位杂交。
iFluor488-dUTP文献参考:
Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77
Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.
Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe
Journal: TheScientificWorldJournal (2012): 609597
SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and K?lvraa, Steen and Nielsen, Kirsten Vang and Nex?, Bj?rn A
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6
[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].
Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai
Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90
Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
Journal: Biotechnology and bioengineering (2003): 127-33
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