酶标记可以通过酶组织化学方法通过产生颜色的反应(色原反应)或产生荧光的反应(荧光反应)来可视化。最常用的酶是辣根过氧化物酶(HRP)。HRP可以通过色原反应(如二氨基联苯胺,例如DAB)、荧光(例如ADHP)或化学发光(例如鲁米诺)来可视化。高度纯化和交叉吸收纯化的IgG在中性条件下与HRP高效率偶联,以最大限度地保留IgG和HRP的活性,使用AAT Bioquest专有的Buccutite?蛋白交联技术。
图:用泛角蛋白小鼠单克隆抗体标记石蜡包埋的人肺癌的免疫荧光图像,随后用HRP标记的山羊抗小鼠IgG(H+L)标记(Cat#16728)。该信号是用AAT的iFluor?488酪胺(目录号11060,绿色)产生的。细胞也用DAPI(蓝色)复染。
艾美捷AAT Bioquest-HRP标记的山羊抗小鼠IgG(重链+轻链):
单位大小:1 毫克
目录编号:16728
分子量:~150000
溶剂:水
H-短语:H303, H313, H333
危害符号:XN
预期用途:仅限研究使用(RUO)
R-短语:R20, R21, R22
储存:冷冻(< -15°C);尽量减少光照
UNSPSC:12171501
AAT Bioquest-HRP标记的山羊抗小鼠IgG(重链+轻链)文献参考:
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