Halo-Flipper is a fluorescent probe that specifically labels Halo-tag™* and reports membrane tension changes through its fluorescence lifetime changes. It contains a Chloroalcane Halo-tag™* ligand as well as a tethered Flipper fluorophore which senses changes of the organization of lipid bilayer membranes surrounding the Halo-tag™* protein . Halo-Flipper spontaneously labels Halotag expressing cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. It is the perfect tool to precisely localize the Flipper-TR membrane tension fluorophore within cells. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of Halo-Flipper (lyophilized) *Halo-tag™ is a registered trademark of Promega Corporation.
