IgM (immunoglobulin M) is the is the third most abundant and largest antibody in the human circulatory system. IgM is expressed on the surface of immature and mature B cells as a monomer and is expressed in a secreted form as a pentamer with very high avidity. IgM is the first human immunoglobulin to appear in response to initial exposure to an antigen and eliminates pathogens in the early stages of B cell-mediated (humoral) immunity before there is sufficient IgG. IgM is a strong complement activator and agglutinator due to its pentameric structure and binds fragment crystallization (Fc) receptors. IgM has a molecular mass of approximately 970 kDa in its pentamer form with ten binding sites. Typically, however, IgM cannot bind 10 antigens at the same time because the large size of most antigens hampers binding to neighboring sites. IgM antibodies do not pass across the human placenta. IgM antibodies are very useful in the diagnosis of infectious diseases. The presence of IgM antibodies in a patient’s serum indicates recent infection, or in a neonate’s serum specifies an intrauterine infection (e.g. congenital rubella).
产品描述
Human IgM ELISA kit is designed for the quantitative detection of human IgM in serum, plasma, and hybridoma cell supernatants.
See kit insert for complete instructions. Store coating buffer, wash buffer, blocking buffer, standard, and reagent diluent at 4˚C. Store substrate solution at 4°C prior to opening. Protect from moisture and light. Store detection antibody and SA-HRP conjugate vial at 4° C prior to restoration. Aliquot capture antibody and target protein standard and freeze at -20˚C. For extended storage aliquot contents and freeze at -20° C or below. Avoid multiple freeze-thaw cycles.
