ATP Kit SL is used to assay both enzymes and metabolites. Any reaction possible to couple to ATP (adenosine triphosphate) formation or degradation, e.g. kinases and their substrates or mitochondrial ATP production, is possible to assay with the ATP Kit SL. ATP Kit SL contains ATP Reagent SL, a reagent emitting a stable light with a decay rate around 0.9 %/min. The stable light makes it possible to continuously monitor the ATP levels in a sample. At the end of the assay the measurement is calibrated with BioThemas internal ATP Standard. Both kinetic and end-point assays can be performed using the 144-041 ATP Kit SL.nnThe kit is intended for monitoring of ATP over the range of 10-12 to 10-6 mol/L. The low decay rate of the light emission is due to a low consumption of ATP in the firefly luciferase reaction and a luciferase activity that is unchanged during the time of the measurement. Under these circumstances measurement of the intensity of the light emission allows continuous monitoring of formation or degradation of ATP in enzymatic reactions or release of ATP from cells. Thus assays can be performed in much the same way as NAD(P)H is spectrophotometrically or fluorometrically monitored in assays based on dehydrogenase reactions. Furthermore the stable light makes assays even of a constant ATP level more convenient and reliable, particularly when using manual luminometers.nnIf a higher sensitivity is required BioThema has two other reagents allowing the detection of 10-17 mol and 10-18, respectively.nnIf no immediate reaction can be coupled to the metabolism of ATP, e.g. pyrosequencing or measurement of DNA polymerase, please contact us at info(at)biothema.com for recommendations of assay development.nnFor ATP determinations in eukaryotic cells we recommend Cell Viability Kit SL or Cellular ATP Kit HTS.nnFor ATP determinations in prokaryotic cells we recommend ATP Biomass Kit HS, Intracellular ATP Kit HS or Microbial ATP Kit HS, which all contain a more sensitive ATP reagent.
产品描述
ATP Kit SL is intended for assays of enzymes and metabolites by monitoring the formation or degradation of ATP (adenosine triphosphate) over the range of 10 –12 to 10 –6 mol/L.nNo. of determinations (cuvettes): 200nnNo. of determinations (microplate): 1000nnDetection limit: Quantitative detection of ATP in the range of 10–12 to 10-6 mol/L.nnDepending of the assay format this means 10–15 to 10–10 mol per assay (1 ml cuvette volume) for tube luminometers and 0.2*10–15 to 0.2*10–10 mol per assay (0.2 ml volume) for microplate luminometers.
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BioThema成立于1987 年，总部位于瑞典斯德哥尔摩南郊，只做一件事：把萤火虫荧光素酶反应做到极致。38 年来，公司依旧保持“小而专”的规模，却凭这一件事成为全球 ATP 与生物冷光分析的隐形冠军。nBioThema致力于萤火虫荧光素酶反应的分析应用。这种多功能反应已广泛用于各种检测，包括细胞数量定量、细胞增殖、细胞毒性、参与ATP形成或降解反应的所有酶和代谢物分析、报告基因检测、免疫检测、快速细胞计数和环境卫生监测。同时BioThema也是全球很多品牌的ATP和荧光素的OEM原料供应商；
