Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many otheranimals, they serve to break down alcohols which could otherwise be toxic; in yeast andmany bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. The Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (lambda = 450nm). This assay detects ADH activity as low as 0.01mU in samples.Kit Components:ADH *Assay Buffer 1x25mlIsopropanol 1x1mlDeveloper (Lyophilized) 1x1vialADH Positive Control (Lyophilized) 1x1vialNADH Standard (0.5umol, Lyophilized) 1x1vial.Storage and Stability:Store the kit at -20°C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
