Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight™ antibody and reagent conjugates are specifically designed for protein detection methods that covers much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight™conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
应用类型
FLISA,IF Microscopy,Western Blot,
免疫原
Fluorescein conjugated to Goat IgG
来源宿主
Goat
反应性
该Anti-FLUORESCEIN (GOAT) Antibody DyLight™ 488 Conjugated - 600-141-096的详细信息查看Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight™ antibody and reagent conjugates are specifically designed for protein detection methods that covers much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight™conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.提供的产品说明书。
保存建议
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
