Cell death （especially apoptosis） is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions，the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore，loss of ATP level in cell has been used as an indicator of cell death. In contrast，cell proliferation has been recognized by increased levels of ATP.Intended Use：Bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells.Specificity：MammalianTest Principle：The Cell Viability BioAssay Kit utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin，and the light can be measured using a luminometer or Beta Counter.Kit Components：Nucleotide Releasing Buffer，20ml，200 AssaysNucleotide Releasing Buffer，100ml，1000 AssaysATP Monitoring Enzyme，1xvial，200 AssaysATP Monitoring Enzyme，5xvials，1000 AssaysEnzyme Reconstitution Buffer，2ml，200 AssaysEnzyme Reconstitution Buffer，5x2ml，1000 AssaysATP，1mg，200 AssaysATP，1mg ,1000 AssaysStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
