Adenylate Kinase （AK） （EC 2.7.4.3） is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. The enzyme is found in the nucleus，cytosol，or mitochondria （intermembrane space or matrix） of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis.Intended Use：Detection of Adenylate Kinase activitySample Type：Purified Adenylate KinaseCell and tissue lysateMitochondrial lysateTest Principle：The AK Activity BioAssay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate （ATP） generated from adenosine diphosphate （ADP） as a substrate. ATP is detected via a multi-step reaction，resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming an end product that can be measured colorimetrically （OD 570 nm） or fluorometrically （Ex/Em = 535/587 nm）.Unit Definition： One unit of Adenylate Kinase activity is the amount of enzyme that generates 1umol of ATP/min. under the assay conditions.Kit Components：AK Assay Buffer，1x25mlAK Probe，1x200ulADP Substrate，1x200ulAK Convertor，1x1vialAK Developer，1x1vialPositive Control （AK Enzyme），1x1vialATP Standard （1 umol），1x1vialStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.
