Pancreatic ribonuclease is an endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. It acts on single-stranded and double-stranded RNA. This ribonuclease can destabilize or unwind the DNA helix by complexing with single-stranded DNA; this complex arises by an extended multisite cation-anion interaction between the lysine and arginine residues of the enzyme and the phosphate groups of the nucleotides. It has endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
应用类型
ELISA,Immunoprecipitation,Western Blot,
免疫原
Ribonuclease A [Bovine pancreas]
来源宿主
Rabbit
反应性
Bovine (Bos Taurus)
保存建议
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
