Anti-IKK gamma detects human IKKgamma. NF-kappaB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kappaB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKKalpha, IKKbeta, and IKKgamma (NEMO). NEMO (NF-kB Essential MOdulator) preferentially interacts with IKK2/b and is required for activation of IKK complex. Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKalpha nor IKKbeta, but complexes directly with NEMO. This suggests that NEMO may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb. Anti-IKKgamma antibody is ideal for investigators involved in NFkappaB, kinase and growth factor research.
应用类型
Flow Cytometry,Western Blot,
免疫原
IKK gamma Antibody was produced in mice prepared by repeated immunizations with human IKKgamma (NEMO) protein at full-length.
来源宿主
Mouse
反应性
H. sapiens (Human)
保存建议
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
