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ImmunoChemistry Magic Red 轻松检测细胞内组织蛋白酶B(Cathepsin B)活力

发布者:艾美捷科技    发布时间:2022-11-29     
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       组织蛋白酶B (Cathepsin B, CTSB)是一种广泛表达于溶酶体上的半胱氨酸肽链内切酶,属于木瓜蛋白酶家族的成员,广泛存在于从病毒到哺乳动物和人等生物体内。可通过参与细胞凋亡、细胞外基质降解、抗原呈递、炎症反应、细胞自噬等多种病理生理过程从而参与多种疾病的发生和发展。   


组织蛋白酶B


       市面上常见的是检测细胞裂解液中的组织蛋白酶B活性,更多研究者希望动态检测 活细胞 内的组织蛋白酶B的活性,做更真实的实验!艾美捷科技为您推荐Immunochemistry Tech(ICT)品牌专利Magic Red系列产品。 

       

       ICT的Magic Red?检测法使研究人员能够在体外对细胞内的蛋白酶活性进行定量和监测。


货号名称规格样本
ICT-937Magic Red Cathepsin-B Assay Kit 魔力红组织蛋白酶B活性分析试剂盒25T培养的活细胞 (悬浮和贴壁)
ICT-938100T

* 仅供科研使用,不得用于医疗. 

       

       【原理】Magic Red魔力红底物,是细胞渗透性的,它很容易穿透细胞膜和内部细胞器的膜——不需要裂解或渗透步骤。如果组织蛋白酶是活性的,魔红底物被裂解,甲酰紫荧光团将在激发时变为荧光(Ex/Em =592nm/628nm)。随着酶活性的进展和更多魔红底物的裂解,信号将增强,使研究人员能够观察颜色随时间的变化。样品可通过荧光显微镜荧光酶标仪进行分析。试剂盒中包括Hoechst 33342和吖啶橙,分别用于检测核形态和溶酶体细胞器结构。   


       【产品组分】

Magic Red Cathepsin-B Assay Kit 魔力红组织蛋白酶B活性分析试剂盒
25T100T
l Magic Red Substrate (MR-RR2), 1 25 Test Vial, #6133 l Hoechst 33342, 1 mL, #639 l Acridine Orange, 0.5 mL, #6130 l Kit Manuall Magic Red Substrate (MR-RR2), 1 100 Test Vial, #6134 l Hoechst 33342, 1 mL, #639 l Acridine Orange, 0.5 mL, #6130 l Kit Manual
保存条件:2-8°C 避光保存。
点击下载说明书PDF

 

        【Nature文章鉴赏】 

        IF=28.2, 《Lysosomal retargeting of Myoferlin mitigates membrane stress to enable pancreatic cancer growth》,Nature Cell Biology 


魔力红?组织蛋白酶B活性分析试剂盒

魔力红?组织蛋白酶B活性分析试剂盒


       Magic Red?相关产品列表:

货号品牌名称规格(T)
ICT-935ImmunochemistryMagic Red Caspase 3 & 7 Assay Kit25
ICT-936ImmunochemistryMagic Red Caspase 3 & 7 Assay Kit100
ICT-937ImmunochemistryMagic Red Cathepsin B Assay Kit25
ICT-938ImmunochemistryMagic Red Cathepsin B Assay Kit100
ICT-939ImmunochemistryMagic Red  Cathepsin K Assay Kit25
ICT-940ImmunochemistryMagic Red  Cathepsin K Assay Kit100
ICT-941ImmunochemistryMagic Red Cathepsin L Assay Kit25
ICT-942ImmunochemistryMagic Red Cathepsin L Assay Kit100

 

       【Magic Red Cathepsin-B Assay Kit,更多发表文章,28+】 

       1、Hao, W;Dian, M;Zhou, Y;Zhong, Q;Pang, W;Li, Z;Zhao, Y;Ma, J;Lin, X;Luo, R;Li, Y;Jia, J;Shen, H;Huang, S;Dai, G;Wang, J;Sun, Y;Xiao, D. Autophagy induction promoted by m6A reader YTHDF3 through translation upregulation of FOXO3 mRNA. Nature communications. 2022 October 4; doi: 10.1038/s41467-022-32963-0. Article   

       2、Li, Y;Xu, B;Ren, X;Wang, L;Xu, Y;Zhao, Y;Yang, C;Yuan, C;Li, H;Tong, X;Wang, Y;Du, J. Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62-Keap1-NRF2 pathway. Cellular & molecular biology letters. 2022 September 30; doi: 10.1186/s11658-022-00383-z. Full Abstract   

       3、Iyer, H;Shen, K;Meireles, AM;Talbot, WS. A lysosomal regulatory circuit essential for the development and function of microglia. Science Advances. 2022 September 2; doi: 10.1126/sciadv.abp8321. Abstract  

       4、Akter, E;Tasaki, Y;Mori, Y;Nakai, K;Hachiya, K;Lin, H;Konno, M;Kamasaki, T;Tanabe, K;Umeda, Y;Yamano, S;Fujita, Y;Kon, S. Non-degradable autophagic vacuoles are indispensable for cell competition. Cell reports. 2022 August 30; doi: 10.1016/j.celrep.2022.111292. Article   

       5、Fernandez, MA;Bah, F;Ma, L;Lee, Y;Schmidt, M;Welch, E;Morrow, EM;Young-Pearse, TL. Loss of endosomal exchanger NHE6 leads to pathological changes in tau in human neurons. Stem cell reports. 2022 August 17; doi: 10.1016/j.stemcr.2022.08.001. Full Report   

       6、Ratto, E;Chowdhury, SR;Siefert, NS;Schneider, M;Wittmann, M;Helm, D;Palm, W. Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly. Nature communications. 2022 August 17; doi: 10.1038/s41467-022-32515-6. Article   

       7、Oe, Y;Kakuda, K;Yoshimura, SI;Hara, N;Hasegawa, J;Terawaki, S;Kimura, Y;Ikenaka, K;Suetsugu, S;Mochizuki, H;Yoshimori, T;Nakamura, S. PACSIN1 is indispensable for amphisome-lysosome fusion during basal autophagy and subsets of selective autophagy. PLoS Genetics. 2022 Jun 1; doi: 10.1371/journal.pgen.1010264. Text  

       8、Hu, M;Li, P;Wang, C;Feng, X;Geng, Q;Chen, W;Marthi, M;Zhang, W;Gao, C;Reid, W;Swanson, J;Du, W;Hume, RI;Xu, H. Parkinson's disease-risk protein TMEM175 is a proton-activated proton channel in lysosomes. Cell. 2022 June 23; doi: 10.1016/j.cell.2022.05.021. Full Text   

       9、Tanno, T;Zhang, P;Bailey, C;Wang, Y;Ittiprasert, W;Devenport, M;Zheng, P;Liu, Y. A novel aptamer-based small RNA delivery platform and its application to cancer therapy. Genes & Diseases. 2022 May 1; doi: 10.1016/j.gendis.2022.05.004. Full Text   

       10、Zhong, W;Rao, Z;Xu, J;Sun, Y;Hu, H;Wang, P;Xia, Y;Pan, X;Tang, W;Chen, Z;Zhou, H;Wang, X. Defective mitophagy in aged macrophages promotes mitochondrial DNA cytosolic leakage to activate STING signaling during liver sterile inflammation. Aging Cell. 2022 May 22; doi: 10.1111/jcpe.13647. Abstract   

       11、Browning, CL;Green, A;Gray, EP;Hurt, R;Kane, AB. Manganese dioxide nanosheets induce mitochondrial toxicity in fish gill epithelial cells. Nanotoxicology. 2021 Jan 27; 1-18. doi: 10.1080/17435390.2021.1874562. Article   

       12、Lan J, Luo H, Wu R, Wang J, Zhou B, Zhang Y, Jiang Y, Xu J. Internalization of HMGB1 (High Mobility Group Box 1) Promotes Angiogenesis in Endothelial Cells. Arterioscler Thromb Vasc Biol. 2020 Dec;40(12):2922-2940. doi: 10.1161/ATVBAHA.120.315151. Epub 2020 Oct 1. Full Text  

       13、J?ger E, Murthy S, Schmidt C, Hahn M, Strobel S, Peters A, St?ubert C, Sungur P, Venus T, Geisler M, Radusheva V, Raps S, Rothe K, Scholz R, Jung S, Wagner S, Pierer M, Seifert O, Chang W, Estrela-Lopis I, Raulien N, Krohn K, Str?ter N, Hoeppener S, Sch?neberg T, Rossol M, Wagner U. Calcium-sensing receptor-mediated NLRP3 inflammasome response to calciprotein particles drives inflammation in rheumatoid arthritis. Nat Commun. 2020 Aug 25;11(1):4243. doi: 10.1038/s41467-020-17749-6. Full Text  

       14、Yin Q, Jian Y, Xu M, Huang X, Wang N, Liu Z, Li Q, Li J, Zhou H, Xu L, Wang Y, Yang C. CDK4/6 regulate lysosome biogenesis through TFEB/TFE3. J Cell Biol. 2020 Aug 3;219(8):e201911036. doi: 10.1083/jcb.201911036. Full Text   

       15、Almacellas E, Pelletier J, Day C, Ambrosio S, Tauler A, Mauvezin C. Lysosomal degradation ensures accurate chromosomal segregation to prevent chromosomal instability. Autophagy. 2020 Jun 23;1-18. doi: 10.1080/15548627.2020.1764727. Online ahead of print. Full Text   

       16、Song SB, Hwang ES. High Levels of ROS Impair Lysosomal Acidity and Autophagy Flux in Glucose-Deprived Fibroblasts by Activating ATM and Erk Pathways. Biomolecules. 2020 May 13;10(5):761. doi: 10.3390/biom10050761. Abstract   

       17、He M, Zhang T, Zhu Z, Qin S, Wang H, Zhao L, Zhang X, Hu J, Wen J, Cai H, Xin Q, Guo Q, Lin L, Zhou B, Zhang H, Xia G, Wang C. LSD1 contributes to programmed oocyte death by regulating the transcription of autophagy adaptor SQSTM1/p62. Aging Cell. 2020 Mar;19(3):e13102. doi: 10.1111/acel.13102. Epub 2020 Feb 19. Full Text   

       18、Cannata Serio M, Graham LA, Ashikov A, Larsen LE, Raymond K, Timal S, Le Meur G, Ryan M, Czarnowska E, Jansen JC, He M, Ficicioglu C, Pichurin P, Hasadsri L, Ríos-Ocampo WA, Gilissen C, Rodenburg R, Jonker JW, Holleboom AG, Morava E, Veltman JA, Socha P, Stevens TH, Simons M, Lefeber DJ. sMutations in the V-ATPase assembly factor VMA21 cause a congenital disorder of glycosylation with autophagic liver disease. Hepatology. 2020 Mar 7. doi: 10.1002/hep.31218. [Epub ahead of print]. Abstract   

       19、Kratschmer C, Levy M. Targeted Delivery of Auristatin Modified Toxins to Pancreatic Cancer Using Aptamers. Molecular Therapy-Nucleic Acids. 2017. 10:227-236 March 2018. doi.org/10.1016/j.omtn.2017.11.013. Full text   

       20、Hover S, Foster B, Fontana J, Kohl A, Goldstein SAN, Barr JN, Mankouri J. Bunyavirus requirement for endosomal K+ reveals new roles of cellular ion channels during infection. PLoS Pathog. 2018. Jan 19;14(1):e1006845. doi: 10.1371/journal.ppat.1006845. eCollection 2018 Jan. Full text   

       21、Festa BP, Chen Z, Berquez M, Debaix H, Tokonami N, Prange JA, Hoek GV, Alessio C, Raimondi A, Nevo N, Giles RH, Devuyst O, Luciani A. Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney. Nat Commun. 2018. Jan 11;9(1):161. doi: 10.1038/s41467-017-02536-7. Full text   

       22、Qian Q, Zhang Z, Orwig, A, Chen S, Ding WX, Xu Y, Kunz RC, Lind NRL, Stamler JS, Yang L. S-nitrosoglutathione Reductase Dysfunction Contributes to Obesity-Associated Hepatic Insulin Resistance via Regulating Autophagy. Diabetes. 2017. Oct 26. pii: db170223. doi: 10.2337/db17-0223. [Epub ahead of print]. Abstract  

       23、Chen J, Ou Y, Li Y, Hu S, Shao LW, Liu Y. Metformin extends C. elegans lifespan through lysosomal pathway. Elife. 2017.Oct 13;6. pii: e31268. doi: 10.7554/eLife.31268. Full Text  

       24、Ríos-Luci C, García-Alonso S, Díaz-Rodríguez E, Nadal-Serrano M, Arribas J, Oca?a A, Pandiella A. Resistance to the antibody-drug conjugate T-DM1 is based in a reduction in lysosomal proteolytic activity. Cancer Res. 2017 Jul 7. doi: 10.1158/0008-5472.CAN-16-3127. [Epub ahead of print]. Abstract.   

       25、Kang HT, Park JT, Choi K, Kim Y, Choi HJC, Jung CW, Lee YS, Chul Park S. Chemical screening identifies ATM as a target for alleviating senescence. Nat Chem Biol. 2017. Jun;13(6):616-623. Epub 2017 Mar 27. Abstract.   

       26、Sakamaki JI, Wilkinson S, Hahn M, Tasdemir N, O’Prey J, Clark W, Hedley A, Nixon C, Long JS, New M, Van Acker T, Tooze SA, Lowe SW, Dikic I, and Ryan KM. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol. Cell. 2017. May 18;66(4):517-532.e9. doi: 10.1016/j.molcel.2017.04.027. Full text.   

       27、Hamanaka T, Nishizawa K, Sakasegawa Y, Oguma A, Teruya K, Kurahashi H, Hara H, Sakaguchi S, Doh-Ura K. Melanin or melanin-like substance interacts with the N-terminal portion of prion protein and inhibits abnormal prion protein formation in prion-infected cells. J Virol. 2017. Abstract.  

       28、Fujita N, Huang W, Lin TH, Groulx JF, Jean S, Nguyen J, Kuchitsu Y, Koyama-Honda I, Mizushima N, Fukuda M, Kiger AA. Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife. 2017. Full text.   


       美国ICT除了可提供全面的细胞活性检测相关试剂,包括凋亡、 caspase、 自噬、细胞毒性、坏死等相关检测,还可以同时提供高质量高灵敏度的 ELISA以及相关试剂,包括包被液、封闭液、 冲洗液、 底物、 终止液、 板子等等。    

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