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worthington 其他组织分离指南

发布者:艾美捷科技    发布时间:2022-06-07     
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原代细胞分离中,针对其他组织涉及到髓核,肌腱细胞、牙髓干细胞、口腔上皮等等的解离通常会使用酶,如何选择合适的酶来完成对应其他组织的解离,以及原代细胞采集的常规操作, 艾美捷针对其他相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:其它组织分离指南

 

本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南

其他组织
种属种属详情细胞名称酶组合推荐,含推荐浓度培养基参考文献
Bovine髓核 / Nucleus pulposusCollagenase Type 2: 0.2%DMEMLee, J., Cheung, K. and Leung, V.: Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability., J Orthop Res 33, 1743-55, 2015
Equine肌腱细胞 / Tendon CellsCollagenase Type 1: 0.1%DMEMNemoto, M., Kizaki, K., Yamamoto, Y., Oonuma, T. and Hashizume, K.: Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration., J Equine Sci 24, 17-24, 2013
山羊Goat髌下脂肪垫间充质基质细胞 / Infrapatellar fat pad mesenchymal stromalCollagenase: 0.15%DMEMArora, A., Sriram, M., Kothari, A. and Katti, D.: Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering., Cytotherapy 19, 881-894, 2017
Human粘附成纤维细胞 / Adhesion fibroblastsCollagenase Type 4: 0.2%Medium 199Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
Human髓核 / Nucleus pulposusPronase: 0.25%;
Collagenase Type 2: 600 u/ml
DMEMSun, Y., Lv, M., Zhou, L., Tam, V., Lv, F., Chan, D., Wang, H., Zheng, Z., Cheung, K. and Leung, V.: Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation., Osteoarthritis Cartilage 23, 1194-203, 2015
Human, 3-7 year牙髓干细胞 / Dental pulp stem cellsCollagenase Type 1: 0.3%;
Neutral Protease: 0.4%
DMEMSalmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE 8, e56749, 2013
Human牙髓干细胞 / Dental pulp stemCollagenase Type 1: 0.3%;
Neutral Protease: 0.4%
DMEM/F12Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol 4, 357, 2013
Human间充质基质细胞 / Mesenchymal stromalCollagenase Type 1: 0.075%PBSLee, Y., Lee, J., Park, H., Lim, Y., Lee, J., Wang, S. and Lee, B.: Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue., Cell Physiol Biochem 31, 513-24, 2013
Human菌状味乳头 / Fungiform taste papillaeCollagenase Type 1: 550 u/ml;
Elastase: 10 u/ml;
Soybean Trypsin Inhibitor: 0.09%
see referenceOzdener, H., Spielman, A. and Rawson, N.: Isolation and Culture of Human Fungiform Taste Papillae Cells, J Vis Exp 63, e3730, 2012
Human肌腱细胞 / Tendon cellsCollagenase Type 2: 0,3%DMEM/Hams F12Pauly, S., Klatte, F., Strobel, C., Schmidmaier, G., Greiner, S. and Scheibel, M. Wildemann B: Characterization of Tendon Cell Cultures of the Human Rotator Cuff., Eur Cell Mater 20, 84-97, 2010
Human脂肪来源的基质细胞 / Adipose derived stromalCollagenase Type 2: 0.075%DMEMPanetta, N., Gupta, D., Kwan, M., Wan, D., Commons, G. and Longaker, M.: Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells., Plast Reconstr Surg 124, 65, 2009
Human口腔上皮 / Oral epithelialNeutral Protease: 1.2 u/ml;
Trypsin: 0.05%
PBSNakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25, 628-38, 2007
Human颈动脉斑块巨噬细胞 / Carotid artery plaque macrophageCollagenase Type 4: 450 u/ml;
Deoxyribonuclease I: 500 u/ml;
Soybean Trypsin Inhibitor: 0.1%
HBSSPatino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM: Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin, Circ Res 98, 1282-9, 2006
Human食管微血管内皮 / Esophageal microvascular endothelialCollagenase Type 2: 0.2%MCDB-131Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003

胃上皮细胞 / Gastric epithelial cellsCollagenase Type 2: 200 u/ml;
Neutral Protease: 1.2 u/ml;
Soybean Trypsin Inhibitor: 0.125%
L-15Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22, 133, 2000
Human滑膜细胞 / SynoviocytesCollagenase: 0.2%DMEM/F12Chen V, Croft D, Purkis P, Kramer IM: Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release., Br J Rheumatol 37, 148-56, 1998
Human消化细胞 / Peptic cellsCollagenase Type 4: 0.1%;
Soybean Trypsin Inhibitor: 0.2%
Ringer solutionLanas. A., Nerin, J., Esteva, F. and Sainz, R.: Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells., Gut 36, 657-63, 1995
Human根尖肉芽肿 / Periapical granulomaCLSPA: 0.25%RPMI-1640Stern MH, Dreizen S, Mackler BF, Levy BM: Isolation and characterization of inflammatory cells from the human periapical granuloma, J Dent Res 61, 1408-12, 1982
昆虫Insect, Lepidoptera鳞翅目 / LepitopteranCollagenase Type 3: 0.35%;
Hyaluronidase: 0.01%
Dulbecco PBSGoodwin, R and McCawley, P: Initiating Attached Cell Lines From the Lepidoptera (Insecta), Meth Cell Sci 3, 567, 1977
无脊椎动物Squid地幔 / MantleCollagenase: see reference;
Papain: see reference
Phosphate bufferRaman, M. and Mathew, S.: Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny., J Food Sci Technol 51, 1509-16, 2014
小鼠Mouse类器官培养物 / Organoid culturesStemxyme 1: 0.1%PBSHuang, L., Desai, R., Conrad, D., Leite, N., Akshinthala, D., Lim, C., Gonzalez, R., Muthuswamy, L., Gartner, Z. and Muthuswamy, S.: Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids.,Cell Stem Cell Vol. 28,, , 1090-1104.e6, 2021
小鼠Mouse, embryonic气管 / TracheaElastase: 0.1%;
Neutral Protease: 0.25%;
Deoxyribonuclease I: 0.01%;
Trypsin: 0.125%
DMEM/F12He, M., Wu, B., Ye, W., Le, D., Sinclair, A., Padovano, V., Chen, Y., Li, K., Sit, R., Tan, M., Caplan, M., Neff, N., Jan, Y., Darmanis, S. and Jan, L.: Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway., Elife 9, 53085, 2020
小鼠Mouse粘附成纤维细胞 / Adhesion fibroblastsCollagenase Type 4: 0.2%Medium 199Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
小鼠Mouse, 8-12 week膀胱 / Urinary bladderCollagenase Type 6: 20 u/mlCollagenase Type 1: 50 u/ml;
Collagenase Type 3: 40 u/ml;
Papain: 45 u/ml;
Deoxyribonuclease I: 4 u/ml;
Tyrode solutionAtta, H., Gheinani, A., Wacker, A., Heshmati, Y., Bigger-Allen, A., Lambrinos, G., Gao, Y., Bielenberg, D. and Adam, R.: A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury., J Vis Exp , , 2020
小鼠Mouse尿路上皮 / UrothelialCollagenase Type 1: 0.5%PBSHuang, C., Chen, C. and Shyr, C.: The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer., Cytotherapy 19, 1233-1245, 2017
小鼠Mouse骨髓基质细胞 / Bone marrow stromalCollagenase Type 1: 0.2%PBSIshida, T., Suzuki, S., Lai, C., Yamazaki, S., Kakuta, S., Iwakura, Y., Nojima, M., Takeuchi, Y., Higashihara, M., Nakauchi, H. and Otsu, M.: Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells., Stem Cells 35, 989-1002, 2017
小鼠Mouse耳蜗 / CochlearPapain: 20 u/ml;
Trypsin: 0.05%
DMEMKim, Y., Wang, S., Tymanskyj, S., Ma, L., Tao, H. and Zhang, L.: Dcc Mediates Functional Assembly of Peripheral Auditory Circuits., Sci Rep 6, 23799, 2016
小鼠Mouse上皮干细胞 / Epithelial stemCollagenase: 2%DMEM/F12Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor., J Vis Exp , , 2014
小鼠Mouse, 26-30 g肌间丛 / Myenteric plexusCollagenase Type 2: 0.13%Neurobasal ASmith, T., Grider, J., Dewey, W. and Akbarali, H.: Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels., PLoS ONE 7, e45251, 2012
小鼠Mouse, 10 week滑膜间充质 / Synovial mesenchymalCollagenase: 0.1%;
Deoxyribonuclease I: 0.005%
DMEMFutami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE 7, e45517, 2012


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