Epigentek：5hmC-羟化酶TET活性/抑制分析试剂盒

5-羟甲基胞嘧啶（5-hydroxymethylcytosine，5-HT）作为第六种DNA碱基，在人类和小鼠的脑细胞和胚胎干细胞中含量丰富，具有转录调控功能。在哺乳动物中，它可以通过5-甲基胞嘧啶的氧化产生，这是由胞嘧啶加氧酶的10 - 11易位（TET）家族介导的反应。

胞嘧啶加氧酶的TET家族包括TET 1、TET 2和TET 3。这些泰特蛋白可以通过结合到富含CpG的区域以防止不需要的DNA甲基转移酶活性，以及通过将5 mC转化为5 hmC并通过羟化酶活性进一步转化为5caC（5-羧基胞嘧啶）来促进DNA去甲基化。结果表明，基因组5 hmC水平与泰特羟化酶活性相关。此外，TET 1通过结合ES细胞中的不同靶基因而显示出在转录激活和抑制中具有双重功能。TET 1也是急性髓性白血病中MLL基因的融合伴侣，被认为是一种癌蛋白。发现TET 2在白血病中经常突变，并被认为是肿瘤抑制剂。TET 3被证明在哺乳动物受精卵中的DNA甲基化重编程过程中发挥独特的作用。因此，激活肿瘤抑制泰特酶如TET 2或抑制癌蛋白TET酶如TET 1将在有益于癌症诊断和开发新的基于靶点的癌症治疗剂方面是重要的。

那么如何检测5hmc羟化酶TET的活性呢？

作为专业的生命科学解决方案供应商，艾美捷为您推荐Nature同款方案：

5hmC-羟化酶TET活性/抑制分析试剂盒 优点和特点：

1、5小时比色测定程序，步骤简单，方便快捷。整个过程可在5小时内完成。

2、通过直接检测TET转化的羟甲基化产物直接测量泰特羟化酶活性。

3、创新的试剂盒组成使背景信号极低，并允许检测简单、准确、可靠和一致。

4、可以使用细胞/组织提取物或纯化泰特蛋白，其允许检测TET羟化酶抑制剂在体内和体外的抑制作用。

5、新的测定原理允许实现高灵敏度。活性可以从低至20ng的纯化的TET1羟化酶检测。

6、包括羟甲基化标准品，其允许定量泰特羟化酶的比活性。 

7、条带微孔板形式使得该测定方法灵活，可用于手动或高通量分析（96次测定）。

Nature文章结果：

DOI https://doi.org/10.1038/s41590-020-0764-8

1.TET酶活性分析使用P-3086;  使用艾美捷推荐的P-1032检测整体5hmc水平

更多客户的选择：

Qian H et. al. (March 2021). TET1 promotes RXRα expression and adipogenesis through DNA demethylation.Biochim Biophys Acta Mol Cell Biol Lipids. :158919.

Wulansari N et. al. (February 2021). LIF Maintains mESC Pluripotency by Modulating TET1 and JMJD2 Activity in a JAK2-Dependent Manner.Stem Cells.

Clark DF et. al. (January 2021). Acute high folic acid treatment in SH-SY5Y cells with and without MTHFR function leads to gene expression changes in epigenetic modifying enzymes, changes in epigenetic marks, and changes in dendritic spine densities.PLoS One. 16(1):e0245005.

Ko?odziej-Wojnar P et. al. (December 2020). Alterations in the Genomic Distribution of 5hmC in In Vivo Aged Human Skin Fibroblasts.Int J Mol Sci. 22(1)

Zhou B et. al. (August 2020). The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic-epigenetic reprogramming and resolves inflammatory injury.Nat Immunol.

Rosenberg T et. al. (August 2020). Embryonic Heat Conditioning Induces TET-Dependent Cross-Tolerance to Hypothalamic Inflammation Later in Life.Front Genet. 11:767.

Gu Y et. al. (July 2020). Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.FASEB J.

Aggarwal et. al. (July 2020). Functional succinate dehydrogenase deficiency is a pathognomonic adverse feature of clear cell renal cancer

Sun X. et. al. et. al. (May 2020). AMPK deficiency induces DNA methylation and aggravates colorectal tumorigenesis in 2 AOM/DSS miceResearch Square.

Kaur G et. al. (April 2020). Regulation of DNA methylation signatures on NF-κB and STAT3 pathway genes and TET activity in cigarette smoke extract-challenged cells/COPD exacerbation model in vitro.Cell Biol Toxicol.

Briand J et. al. (April 2020). Radiotherapy-induced overexpression of exosomal miRNA-378a-3p in cancer cells limits natural killer cells cytotoxicity.

Zhou Y et. al. (March 2020). HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p.Cell Death Dis. 11(3):176.

Bhagat TD et. al. (October 2019). Lactate-mediated epigenetic reprogramming regulates formation of human pancreatic cancer-associated fibroblasts.8

Zhou L et. al. (October 2019). TET2-interacting long noncoding RNA promotes active DNA demethylation of the MMP-9 promoter in diabetic wound healing.Cell Death Dis. 10(11):813.

Parsanathan R et. al. (October 2019). Glutathione deficiency induces epigenetic alterations of vitamin D metabolism genes in the livers of high-fat diet-fed obese mice.Sci Rep. 9(1):14784.

Shi F et. al. (April 2019). EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the <i>RIP3</i> promoter.9(9):2424-2438.

Cramer T et. al. (December 2018). PARP Inhibitor Affects Long-term Heat-stress Response via Changes in DNA Methylation.399:65-76.

Cramer T et. al. (October 2018). Early-life epigenetic changes along the corticotropin-releasing hormone (CRH) gene influence resilience or vulnerability to heat stress later in lifeMolecular Psychiatry.

Poupeau A et. al. (October 2018). Genes controlling the activation of natural killer lymphocytes are epigenetically remodeled in intestinal cells from germ-free mice.FASEB J. :fj201800787R.

Atlante S et. al. (July 2018). α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.Cell Death Dis. 9(7):756.

Shahid M et. al. (March 2018). Alpha-oxoglutarate inhibits the proliferation of immortalized normal bladder epithelial cells via an epigenetic switch involving ARID1A.Sci Rep. 8(1):4505.

Rea M et. al. (November 2017). Selective inhibition of CTCF binding by iAs directs TET-mediated reprogramming of 5-hydroxymethylation patterns in iAs-transformed cells.Toxicol Appl Pharmacol. 338:124-133.

Wulansari N et. al. (October 2017). Vitamin C-Induced Epigenetic Modifications in Donor NSCs Establish Midbrain Marker Expressions Critical for Cell-Based Therapy in Parkinson's Disease.Stem Cell Reports. 9(4):1192-1206.

Shenoy N et. al. (July 2017). Upregulation of TET activity with ascorbic acid induces epigenetic modulation of lymphoma cells.Blood Cancer J. 7(7):e587.

Prasad R et. al. (May 2017). Honokiol inhibits ultraviolet radiation-induced immunosuppression through inhibition of ultraviolet-induced inflammation and DNA hypermethylation in mouse skin.Sci Rep. 7(1):1657.

R?hr D et. al. (April 2017). Sodium-dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation.

Kowluru RA et. al. (January 2017). Role of oxidative stress in epigenetic modification of MMP-9 promoter in the development of diabetic retinopathy.Graefes Arch Clin Exp Ophthalmol.

Ishikawa K et. al. (February 2015). Long-term pancreatic beta cell exposure to high levels of glucose but not palmitate induces DNA methylation within the insulin gene promoter and represses transcriptional activity.PLoS One. 10(2):e0115350.

Niu Y et. al. (February 2015). Oxidative stress alters global histone modification and DNA methylation.Free Radic Biol Med.

He XB et. al. (December 2014). Vitamin C Facilitates Dopamine Neuron Differentiation in Fetal Midbrain Through Tet1- aND Jmjd3-Dependent Epigenetic Control Manner.Stem Cells.

Dubois-Chevalier J et. al. (September 2014). A dynamic CTCF chromatin binding landscape promotes DNA hydroxymethylation and transcriptional induction of adipocyte differentiation.Nucleic Acids Res.

Scola G et. al. (April 2014). Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons.Psychopharmacology (Berl).

Nettersheim D et. al. (December 2013). Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development.PLoS One. 8(12):e82881.

Coulter JB et. al. (October 2013). Hydroquinone increases 5-hydroxymethylcytosine formation through ten eleven translocation 1 (TET1) 5-methylcytosine dioxygenase.J Biol Chem. 288(40):28792-800.