为答谢和回馈广大科研工作者们长期以来对武汉艾美捷科技有限公司的大力支持，在新春到来之际，艾美捷科技联合细胞骨架领域顶级供应商 Cytoskeleton 联合推出：小G蛋白&细胞成像产品线新春超值大促，一年仅此一次，千万不要错过啊！本次促销活动产品涵盖 小G蛋白活化检测试剂盒，激动剂、和及Tubulin，Actin，溶酶体，DNA，质膜等细胞组分成像的荧光染料等。
活动详情：
时间：2022年1月15日-2022年2月28日
对象：仅限中国区终端客户
品目：Cytoskeleton小G蛋白&细胞成像相关产品（详情见下方活动品目列表）
内容： 直享 6.5折超低优惠！
活动品目列表：
分类 | 艾美捷货号 | 产品名称 | 规格 | 原价 | 折扣价 |
Actin抗体 | CSK-AAN02 | Anti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1) | 1x500ul | 4316 | 2805 |
CSK-AAN02-S | Anti-Pan Actin Mouse Monoclonal Antibody (Clone 7A8.2.1) | 1x125ul | 1526 | 992 | |
小G蛋白活化检测试剂盒 | CSK-BK001 | Actin Binding Protein Spin-Down Assay Kit | KIT30-100assays | 11637 | 7564 |
CSK-BK003 | Actin Polymerization Biochem Kit | KIT30-100assays | 13255 | 8616 | |
CSK-BK008 | Ras Activation Assay Biochem Kit | KIT50assays | 11251 | 7313 | |
CSK-BK008-S | Ras Activation Assay Biochem Kit | KIT20assays | 5703 | 3707 | |
CSK-BK030 | Combo RhoA/Rac1/Cdc42 Activation Assay Kit | KIT3x10assays | 14873 | 9668 | |
CSK-BK032-S | Arf1 Pulldown Activation Assay Kit | KIT20assays | 6859 | 4458 | |
CSK-BK033-S | Arf6 Pulldown Activation Assay Kit | KIT20assays | 6859 | 4458 | |
CSK-BK034 | Cdc42 Pulldown Activation Assay Kit | KIT50assays | 11251 | 7313 | |
CSK-BK034-S | Cdc42 Pulldown Activation Assay Kit | KIT20assays | 5703 | 3707 | |
CSK-BK035 | Rac1 Pulldown Activation Assay Kit | KIT50assays | 11251 | 7313 | |
CSK-BK035-S | Rac1 Pulldown Activation Assay Kit | KIT20assays | 5703 | 3707 | |
CSK-BK036 | RhoA Pulldown Activation Assay Kit | KIT80assays | 11251 | 7313 | |
CSK-BK036-S | RhoA Pulldown Activation Assay Kit | KIT20assays | 5703 | 3707 | |
CSK-BK037 | G-Actin : F-Actin In Vivo Assay Kit | KIT30-100assays | 11174 | 7263 | |
CSK-BK040 | RalA Activation Assay Biochem Kit | KIT50assays | 11251 | 7313 | |
CSK-BK121 | RhoA G-LISA Activation Assay (luminescence) | KIT96assays | 13794 | 8966 | |
CSK-BK124 | RhoA G-LISA Activation Assay (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK124-S | RhoA G-LISA Activation Assay (colorimetric) | KIT24assays | 8400 | 5460 | |
CSK-BK124-XL | RhoA G-LISA Activation Assay (colorimetric) | 大规格 | 询价 | 询价 | |
CSK-BK125 | Rac1,2,3 G-LISA Activation Assay Kit (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK126 | Rac1 G-LISA Activation Assay Kit | KIT96assays | 13794 | 8966 | |
CSK-BK127 | Cdc42 G-LISA Activation Assay Kit (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK127-S | Cdc42 G-LISA Activation Assay Kit (colorimetric) | KIT24assays | 8400 | 5460 | |
CSK-BK128 | Rac1 G-LISA Activation Assay Kit (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK128-S | Rac1 G-LISA Activation Assay Kit (colorimetric) | KIT24assays | 8400 | 5460 | |
CSK-BK129 | RalA G-LISA Activation Assay Kit (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK131 | Ras G-LISA Activation Assay Kit (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK131-XL | Ras G-LISA Activation Assay Kit | 大规格 | 询价 | 询价 | |
CSK-BK132 | Arf1 G-LISA Activation Assay Kit (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK133 | Arf6 G-LISA Activation Assay Kit (colorimetric) | KIT96assays | 13794 | 8966 | |
CSK-BK135 | G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) (colorimetric) | 3KITS(24assays/kit) | 23273 | 15128 | |
CSK-BK150 | RhoA ELISA Kit (colorimetric) | KIT96assays | 7629 | 4959 | |
小G蛋白相关活化剂及抑制剂 | CSK-CN01-A | Rho Activator | 5x10units | 3237 | 2104 |
CSK-CN01-B | Rho Activator | 20x10units | 11637 | 7564 | |
CSK-CN02-A | Rac and Cdc42 Activator | 5x10units | 3237 | 2104 | |
CSK-CN02-B | Rac and Cdc42 Activator | 20x10units | 11637 | 7564 | |
CSK-CN03-A | Rho activator II | 3x20ug | 3237 | 2104 | |
CSK-CN03-B | Rho activator II | 9x20ug | 9248 | 6011 | |
CSK-CN03-XL | Rho activator II | 大规格 | 询价 | 询价 | |
CSK-CN04-A | Rho/Rac/Cdc42 Activator I | 3x20ug | 3237 | 2104 | |
CSK-CN04-B | Rho/Rac/Cdc42 Activator I | 9x20ug | 9248 | 6011 | |
CSK-CT03-A | C3 transferase protein | 1x25ug | 3237 | 2104 | |
CSK-CT03-C | C3 transferase protein | 4x25ug | 11251 | 7313 | |
CSK-CT04-A | Cell Permeable Rho Inhibitor (C3 Trans based)) | 1x20ug | 3237 | 2104 | |
CSK-CT04-B | Cell Permeable Rho Inhibitor (C3 Trans based)) | 5x20ug | 9864 | 6412 | |
CSK-CT04-C | Cell Permeable Rho Inhibitor (C3 Trans based)) | 20x20ug | 25431 | 16530 | |
Tubulin，Actin，溶酶体，DNA，质膜染料 | CSK-CY-SC001 | SiR-Actin Kit | 50-300slides | 8184 | 5320 |
CSK-CY-SC002 | SiR-Tubulin Kit | 50-300slides | 8184 | 5320 | |
CSK-CY-SC006 | Cytoskeleton Kit (SiR-Actin + SiR-Tubulin) | 50-300slideseach | 13640 | 8866 | |
CSK-CY-SC007 | SiR-DNA Kit | 50-300slides | 4683 | 3044 | |
CSK-CY-SC012 | SiR-Lysosome Kit | 50-300slides | 5965 | 3877 | |
CSK-CY-SC013 | SiR700-Actin Kit | 35-200slides | 8184 | 5320 | |
CSK-CY-SC014 | SiR700-Tubulin Kit | 35-200slides | 8184 | 5320 | |
CSK-CY-SC015 | SiR700-DNA Kit | 35-200slides | 4683 | 3044 | |
CSK-CY-SC016 | SiR700-Lysosome Kit | 35-200slides | 5965 | 3877 | |
CSK-CY-SC020 | Flipper TR membrane tension sensor | 35-200slides | 6196 | 4027 | |
CSK-CY-SC101 | SPY505-DNA Probe | 100slides | 5194 | 3376 | |
CSK-CY-SC201 | SPY555-DNA Probe | 100slides | 5194 | 3376 | |
CSK-CY-SC202 | SPY555-Actin Probe | 100slides | 6951 | 4518 | |
CSK-CY-SC203 | SPY555-Tubulin Probe | 100slides | 6951 | 4518 | |
CSK-CY-SC204 | SPY555-BG Substrate, Abs./Em. 555/580 nm | 35nmoles | 5194 | 3376 | |
CSK-CY-SC301 | SPY595-DNA Probe | 100slides | 5194 | 3376 | |
CSK-CY-SC401 | SPY620-DNA Probe | 100slides | 5194 | 3376 | |
CSK-CY-SC402 | SPY620-Actin Probe | 100slides | 6951 | 4518 | |
CSK-CY-SC404 | SPY620-BG Substrate, Abs./Em. 619/635 nm | 35nmoles | 5194 | 3376 | |
CSK-CY-SC501 | SPY650-DNA Probe | 100slides | 5194 | 3376 | |
CSK-CY-SC503 | SPY650-Tubulin Probe | 100slides | 6951 | 4518 | |
CSK-CY-SC504 | SiR650-BG Substrate, Abs./Em. 652/674 nm | 35nmoles | 5194 | 3376 | |
CSK-CY-SC601 | SPY700-DNA Probe | 100slides | 5194 | 3376 | |
CSK-CY-SC604 | SiR700-BG Substrate, Abs./Em. 696/718 nm | 35nmoles | 5194 | 3376 | |
CSK-MG01-02 | MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499 em 507 50-300 slides (MEMBRIGHT Family Probe) | 50-300slides | 3005 | 1954 | |
CSK-MG01-10 | MemGlow 488 - Fluorogenic Plasma Membrane probe ex 499 em 507 250-1500 slides (MEMBRIGHT Family Probe) | 250-1500slides | 5626 | 3657 | |
CSK-MG02-02 | MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555 em 570 50-300 slides (MEMBRIGHT Family Probe) | 50-300slides | 3005 | 1954 | |
CSK-MG02-10 | MemGlow 560 - Fluorogenic Plasma Membrane probe ex 555 em 570 250-1500 slides (MEMBRIGHT Family Probe) | 250-1500slides | 5626 | 3657 | |
CSK-MG03-02 | MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580 em 620 50-300 slides (MEMBRIGHT Family Probe) | 50-300slides | 3005 | 1954 | |
CSK-MG03-10 | MemGlow 590 - Fluorogenic Plasma Membrane probe ex 580 em 620 250-1500 slides (MEMBRIGHT Family Probe) | 250-1500slides | 5626 | 3657 | |
CSK-MG04-02 | MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650 em 673 50-300 slides (MEMBRIGHT Family Probe) | 50-300slides | 3005 | 1954 | |
CSK-MG04-10 | MemGlow 640 - Fluorogenic Plasma Membrane probe ex 650 em 673 250-1500 slides (MEMBRIGHT Family Probe) | 250-1500slides | 5626 | 3657 | |
CSK-MG05-02 | MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650 em 720 50-300 slides (MEMBRIGHT Family Probe) | 50-300slides | 3005 | 1954 | |
CSK-MG05-10 | MemGlow 700 - Fluorogenic Plasma Membrane probe ex 650 em 720 250-1500 slides (MEMBRIGHT Family Probe) | 250-1500slides | 5626 | 3657 | |
CSK-PHDG1-A | Acti-stain 488 phalloidin | 1x500ul(300slides) | 3005 | 1954 | |
CSK-PHDH1-A | Acti-stain 555 phalloidin | 1x500ul(300slides) | 3005 | 1954 | |
CSK-PHDN1-A | Acti-stain 670 phalloidin | 1x500ul(300slides) | 3005 | 1954 | |
CSK-PHDR1 | Rhodamine Phalloidin (14 uM in methanol) | 1x500ul | 3005 | 1954 |
*本活动最终解释权归艾美捷科技所有
产品应用详情了解：
1.小G蛋白活化检测：
小G蛋白(Small G Protein)因分子量只有20—30KD而得名，同时具有GTP酶活性，在细胞信号通路中发挥分子开关的功能，参与调控很多生物学过程，包括分泌活动和细胞骨架的装配等。目前发现的小G蛋白包括 Rho, Rac, Arf1 & 6, Ras, Ral 等。小G蛋白的共同特点是，当结合了GTP时即成为活化形式，这时可作用于下游分子使之活化，而当GTP水解成为GDP时（自身为GTP酶）则恢复到非活化状态。
Pull-down和G-LISA实验都被广泛地用于研究小G蛋白的活性。Pull-down实验又叫蛋白质体外结合实验，可用于测定活性的GTPase亚型，可作为对G-LISA实验的补充。G-LISA 实验是一种改进的ELISA，可测定3-D细胞系、原代细胞裂解液及组织裂解液中有活性的GTPase，使GTPase定量的准确性增加。
Pull-down及G-LISA实验结果展示：
 Lanes 4 & 5:10 ng/ ml of EGF， 2min Lanes 2 & 3: Persistent serum starvation Lanes 1: 20 ng of recombinant Rac1-His protein run as a western blot standard |
分别用CN01（蓝色Activators）和LPA（红色）处理后RhoA的活化进程 |
2.细胞组分成像结果展示：
Hela细胞有丝分裂，SiR-Tubulin染色Tubulin蛋白（绿色） |
SiR和SiR700 Actin染色活细胞的Actin |
鬼笔环肽染色固定细胞F-Actin |
HeLa细胞有丝分裂延时成像，有丝分裂是一个高度敏感的过程，显示SiR-DNA染料的低细胞毒性。 |
Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。
产品订购：sales@amyjet.com
邮政编码：430070
公司地址：武汉市洪山区光谷大道35号
光谷总部国际二期时代1栋13楼
提示：本公司所有产品仅供科研使用，不用于临床诊断。
版权所有：艾美捷科技有限公司 鄂ICP备10204150号-1 鄂公网安备：42018502004523号
第二类医疗器械经营备案凭证：鄂汉药监械经营备20234324号
微信扫码在线客服
