Fibulin-2, a 1221 amino acids protein containing 26-residue signal peptide, is an extracellular matrix protein expressed by normal epithelia. Study by immunofluorescence localizations using Mouse tissue, Fibulin-2 was extracellular localized in basement membrane and other connective tissue compartments. By Immunohistochemistry, Fibulin-2 was expressed in fibrobasts but not in several tumor cell lines. In the serum level of fibulin-2 is 1,000-fold lower than that of fibulin-1. Fibulin-2 was found to be down-regulated in several breast cancer cell lines as well as the decreased expression in breast cancer tissue samples. Re-introduction of Fibulin-2 into breast cancer cell lines that do not express Fibulin-2 reduced cancer cell motility and invasion in vitro but had no effect on cell growth and adhesion properties. Together with evidence that Fibulin-2 contributes to wound healing and inhibits smooth muscle cell migRation. The loss of Fibulin-2 expression may facilitate migRation and invasion in breast cancer.
应用类型
Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
免疫原
A synthetic peptide corresponding to the 1140-1165aa of Fibulin-2 protein. This sequence is identical to mouse, human and rat
来源宿主
Rabbit
反应性
该Anti-Fibulin 2 Polyclonal 抗体的反应性请参考该产品的说明书
保存建议
The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
其他
Aviva Systems Biology总部位于加利福尼亚州圣迭戈，在中国北京设有办公室，专注于为研究需求提供多克隆和单克隆抗体、ELISA试剂盒、蛋白质和定制服务。Aviva Systems Biology生产了24,000种经过验证的多克隆抗体，并提供近20,000种ELISA试剂盒，定制实验室服务包括蛋白表达和纯化、抗体开发，以及ELISA的开发、验证和生产。Aviva Systems Biology为与独特物种和靶标相关的研究提供独特工具，研究领域包括转录因子、癌症、心血管、细胞生物学、DNA损伤和修复、表观遗传学、信号转导、细胞分化、干细胞生物学等等。
