- 中文名称
Anti- GSK3-beta (Phospho-Tyr216) 抗体
- 英文名字
- Anti- GSK3-beta (Phospho-Tyr216) Antibody
- 供应商
- Aviva Systems Biology
- 产品货号
- aviva-GWB-516365-50UG
- 产品报价
- ￥询价/50ug
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系。本公司所有产品仅供科研使用，不用于临床诊断。
- 产品新闻
- 背景资料
- Product Name: b-HCG
# of Samples: 1 x 96 Assays
Intended Use: Immunoenzymatic colorimetric method (ELISA) for quantitative determination of b-HCG in serum or plasma.
Introduction: Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted in pregnancy, that is made by the embryo soon after conception and later by the syncytiotrophoblast (part of the placenta). Its role is to prevent the disintegration of the corpus luteum of the ovary and thereby maintain progesterone production that is critical for a pregnancy in humans. hCG may have additional functions, for instance it is thought that it affects the immune tolerance of the pregnancy.
Pregnancy tests measure the levels of hCG in the blood or urine to indicate the presence or absence of an implanted embryo. In particular, pregnancy tests employ an antibody that is specific to the β-subunit of hCG (βhCG). This is important so that tests do not make false positives by confusing hCG with LH and FSH.
βhCG is also secreted by some cancers including teratomas, choriocarcinomas. But, elevated levels cannot prove the presence of a tumor, and low levels do not rule it out.
Principles of the assay: The b-HCG assay is based on simultaneous binding of human b-HCG to two monoclonal antibodies; one is immobilized on the microplate, the other is soluble and conjugated with horseradish peroxidase (HRP). Microtiter strip wells are precoated with anti-b-HCG IgG antibodies. b-HCG in samples and standards binds to the immobilised antibodies on the surface of the microtiter wells and the second, soluble anti-b-HCG antibody-enzyme conjugate binds to the immobile antibody-b-HCG-complex during the first incubation. Afterwards a washing step is performed. The immune complex is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of b-HCG in samples and standards. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorption at 450 nm is read using an ELISA microwell plate reader.
Storage and Stability: The closed reagents are stable up to the expiry date stated on the label when stored at 2...8 C.
Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values.
References Wisdom, G.B., Clin. Chem. (1976) 22/8, 1243 – 1255Shome, B. and Parlow, A.F.; J. Clin. Endocr. Metab. (1974) 39, 199 – 202Uotila, M., Ruoslahti, E., Engvall, E.J., J. Immunol. Methds (1981) 42, 11 – 15Acosta, A.A.M.D. and Wright, G.L., Journal of Clinical Immunoassays (1983) 6, 41Cohen, K.L., Metabolism (1977) 26, 1165 – 1177Lindstedt (1982) Scand. J. Clin. Lab. Invest 42, 201Chien (1987) J. Clin. Endocr. Metab. 64, 313
- 应用类型
- 该Anti- GSK3-beta (Phospho-Tyr216) 抗体的应用类型请参考该产品的说明书
- 免疫原
- 该Anti- GSK3-beta (Phospho-Tyr216) 抗体的免疫原请参考该产品的说明书
- 来源宿主
- 该Anti- GSK3-beta (Phospho-Tyr216) 抗体的来源宿主请参考该产品的说明书
- 反应性
- 该Anti- GSK3-beta (Phospho-Tyr216) 抗体的反应性请参考该产品的说明书
- 保存建议
- 该Anti- GSK3-beta (Phospho-Tyr216) 抗体的保存建议请参考该产品的说明书
- 其他
- Aviva Systems Biology总部位于加利福尼亚州圣迭戈，在中国北京设有办公室，专注于为研究需求提供多克隆和单克隆抗体、ELISA试剂盒、蛋白质和定制服务。Aviva Systems Biology生产了24,000种经过验证的多克隆抗体，并提供近20,000种ELISA试剂盒，定制实验室服务包括蛋白表达和纯化、抗体开发，以及ELISA的开发、验证和生产。Aviva Systems Biology为与独特物种和靶标相关的研究提供独特工具，研究领域包括转录因子、癌症、心血管、细胞生物学、DNA损伤和修复、表观遗传学、信号转导、细胞分化、干细胞生物学等等。
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
