中文名称

Secretin Human

英文名字

Secretin Human

供应商

Aviva Systems Biology

产品货号

aviva-GWB-35EB92-1MG

产品报价

￥询价/1mg

产品说明书

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产品新闻

背景资料

Product Name	Estradiol ELISA**
Sample Type	Serum
# of Samples	1x96 well
Intended Use	The GenWay, Inc. Estradiol (E2) ELISA Kit is intended for the quantitative determination of Estradiol (E2) concentration in human serum.
Principle of the test	The GenWay Biotech, Inc E2 ELISA kit is based on the principle of Delayed competitive binding assay between E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti-Estradiol monoclonal antibody epitopes (Biotin reagent). In the incubation, anti-E2 antibody biotin reagent, E2 standards, controls, and samples are incubated for 45 minutes at room temperature (RT), then E2 enzyme conjugate is added on the top of the reaction mixture and incubation continues for 45 minutes more. During the incubation, a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. E2 peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of E2 in the specimen increases. Unbound of anti-Estradiol Biotin Reagent and E2 peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance
Storage and Stability	2-8C.
References	Horm Behav 2013 Jan;63(1):144-57. doi: 10.1016/j.yhbeh.2012.09.011. Epub 2012 Oct 12. 17β-Estradiol, but not estrone, increases the survival and activation of new neurons in the hippocampus in response to spatial memory in adult female rats. McClure RE, Barha CK, Galea LA Department of Psychology, Graduate Program in Neuroscience, Brain Research Centre, University of British Columbia, Vancouver, BC, Canada.

序列

该Secretin Human的序列请参考该产品的说明书

来源宿主

该Secretin Human的来源宿主请参考该产品的说明书

溶解建议

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保存建议

该Secretin Human的保存建议请参考该产品的说明书

其他

Aviva Systems Biology总部位于加利福尼亚州圣迭戈，在中国北京设有办公室，专注于为研究需求提供多克隆和单克隆抗体、ELISA试剂盒、蛋白质和定制服务。Aviva Systems Biology生产了24,000种经过验证的多克隆抗体，并提供近20,000种ELISA试剂盒，定制实验室服务包括蛋白表达和纯化、抗体开发，以及ELISA的开发、验证和生产。Aviva Systems Biology为与独特物种和靶标相关的研究提供独特工具，研究领域包括转录因子、癌症、心血管、细胞生物学、DNA损伤和修复、表观遗传学、信号转导、细胞分化、干细胞生物学等等。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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