X-gal is a substrate of β-galactosidase. It shows a blue color after hydrolyzation. Using this property, when lacZ-deficient cell as a host is transformed with vector DNA carrying lacZ gene, IPTG is added together with X-gal into culture medium, based on the α-complementation of β-galactosidase, recombinant clones can be easily identified upon whether white colonies are observed.
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X-gal is a substrate of β-galactosidase. It shows a blue color after hydrolyzation. Using this property, when lacZ-deficient cell as a host is transformed with vector DNA carrying lacZ gene, IPTG is added together with X-gal into culture medium, based on the α-complementation of β-galactosidase, recombinant clones can be easily identified upon whether white colonies are observed.