- 中文名称
ZO Tight Junction Antibody Kit, BioAssay discontinued
- 英文名字
- ZO Tight Junction Antibody Kit, BioAssay discontinued
- 供应商
- USBiological
- 产品货号
- Z0775
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Tight junctions are specialized regions of cell-cell contact that seal the paracellular space. TJ strands on adjacent cells are presumed to interact with each other to form a molecular gasket that prevents ions, water and other molecules from leaking between cells. In addition to this barrier function, the fence function of tight junctions plays an important role in maintaining epithelial cell polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. At one time, the only transmembrane protein known to be associated with tight junctions was occludin, a ~65kD protein with four transmembrane domains. Despite widespread expectation, a critical structural role for occludin in TJ strands was ruled out by the observation of apparently normal tight junctions formed between cells disrupted at both occludin alleles. Fortunately, a closer examination of isolated tight junctions uncovered two related ~22kD, four-transmembrane domain proteins, claudin-1 and claudin-2, with no similarity to occludin. In contrast to occludin, which induces only a small number of short strands at cell-cell contact sites when introduced into fibroblasts lacking tight junctions, claudin-1 and -2 induce networks of strands characteristic of true tight junctions.1,2 These findings suggest thatclaudin-1 and -2 are major structural components of TJ strands and that occludin plays an accessory role. Claudins interact directly with the membrane-associated guanylate kinase homologues, ZO-1, ZO-2, and ZO-3, and are complexed with two other transmembrane proteins, occludin and junctional adhesion molecule.3 E-Cadherin is also important for the formation and maintenance of tight junctions.4 Cell-cell contacts containing E-Cadherin are crucial to the proper assembly and permanent sealing of tight junctions.5 To date, 20 claudins have been identified; studies suggest that the claudins comprising tight junctions are differentially expressed in different tissues. A number of human diseases are caused by claudin defects, which underscores the importance of claudins in human physiology. Deletion of the claudin-11 gene in mice results in disappearance of tight junction fibrils in testis and male sterility, as well as delayed axonal conduction rates in the CNS.7 An autosomal recessive form of deafness has been associated with human mutations in claudin-14.8 Claudin-7 expression is reportedly lower in invasive ductal breast carcinomas.Kit Components:Z0775A: Ms/Mono; Z0775B: Rb/Poly; Z0775C: Ms/Mono; Z0775D: Rb/Poly; Z0775E: Rb/Poly; Z0775F: Ms/Mono.All Mabs are IgG1, kappa and 20ug. The Pabs are IgG and 10ug.Z0775A, Z0775B: ZO-1; Z0775C, Z0775D: Occludin; Z0775E: Claudin-1; Z0775F: E-CadherinZ0775A: Human, canineZ0775B: Human, mouse, rat, canineZ0775C: Human, rat, canineZ0775D: Human, canineZ0775E: Human, rat, canineZ0775F: HumanApplications:Z0775A: Ms/Mono;Z0775C: Ms/Mono; Z0775F: Ms/Mono. Suitable for use in ELISA, Immunofluorescence and Western BlotZ0775B: Rb/Poly; Z0775D: Rb/Poly; Z0775E: Rb/Poly;Suitable for use in ELISA, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Recommended Dilution: ELISA: 0.1-1ug/mlImmunofluorescence: 10-20ug/mlWestern blot: 0.5-3ug/mlImmunohistochemistry (with ZO-1 and Occludin pAbs and E-Cadherin mAb): 2-5ug/mlImmunoprecipitation (with ZO-1 and Occludin pAbs and E-Cadherin mAb): 2-5ug/ IP reaction.Optimal dilution determined by the researcher.Pretreatment protocols for use with formalin-fixed, paraffin-embedded tissue sections.1. ZO-1 and Occludin pAbs: Protease (Sigma P-5147) at 1-2mg/ml for 10 minutes at 37°C, after slides have been deparaffinized and rehydrated in ethanol. Normal human colon sections may be used as a positive control.2. E-Cadherin mAb: HIER with citrate buffer, pH 6.0 for 15 minutes at 95°C, or boiling. Normal human colon sections may be used a positive control.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- 产品特点
- 针对ZO Tight Junction Antibody Kit, BioAssay discontinued该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到ZO Tight Junction Antibody Kit, BioAssay discontinued产品后将其置于4°C (-20°C Glycerol)保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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