- 中文名称
Western Blot ECL Kit with Enhancer, Anti-Mouse, BioAssay
- 英文名字
- Western Blot ECL Kit with Enhancer, Anti-Mouse, BioAssay
- 供应商
- USBiological
- 产品货号
- W1019-82A
- 产品报价
- ￥23
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Ultra-sensitive ECL Western Blot System that detects attogram (10e18g) or yoctomole (10e24M) protein levels. The Enhancer utilizes a proprietary technology that increases antigen and antibody affinity and accessibility which can work with different antigens and antibodies, such as structural proteins (Actin), enzymes (GAPDH) or membrane proteins (EGFR), increasing Western blot signals dramatically.Kit Components:W1019-82A1: Enhancer, 1x50ml or 1x100mlW1019-82A12 Antibody Binding Buffer, 20x, 1x60ml or 1x120mlW1019-82A3: Blocking Agent, 1x20g or 1x40gW1019-82A4: HRP Conjugated Secondary Antibody (anti-Mouse), 1x50ul or 1x100ulW1019-82A5: Luminescence Substrate solution A, 1x25ml or1x100mlW1019-82A6: Luminescence Substrate solution B, 1x25ml or 1x50mlStorage and Stability:May be stored at 4°C. Stable for 6 months after receipt. Two convenient and affordable sizes:15 Mini Blots (1200cm2) and 30 Mini Blots (2500cm2).Quality Control:Duplicated Western blots containing 10fg and 10pg of antigen are detected with anti-GAPDH antibody. Antigen at 10fg levels was detected only on the blot treated with Enhancer, with similar intensity as antigen at 10pg level on the untreated blot.One ng of purified Rabbit muscle GAPDH was serially diluted to 1 attogram and electrophoresis was performed. The gel was transferred to a PVDF membrane, and then cut in the middle. The right section was treated with Enhancer, and the left was untreated. Immunoblotting was done using a primary antibody and HRP-conjugated rabbit anti-mouse antibody, and chemiluminescent substrates were applied. A film exposed for two minutes is shown above. 10-e11g of antigen without treatment and 10e14g of GAPDH with treatment gave similar signal strengths, indicating a 1000-fold enhancement*.* Fold enhancement varied from a few to a 1000-fold depending on the characteristics of antibodies and antigen used.Note:Components in this kit are free of precipitate. When testing by visible spectrophotometry, the color of the solution is comparable to a control Lot. Results from each lot are comparable to those obtained with control lot of the solution.
- 产品特点
- 针对Western Blot ECL Kit with Enhancer, Anti-Mouse, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Western Blot ECL Kit with Enhancer, Anti-Mouse, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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