- 中文名称
West Nile Virus Protease, Fluorometric 440, BioAssay Kit
- 英文名字
- West Nile Virus Protease, Fluorometric 440, BioAssay Kit
- 供应商
- USBiological
- 产品货号
- W1018-69
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Kit Size: 500 Assays (96-well)This kit is optimized to detect West Nile Virus protease activity. It provides enough reagents to perform 500 assays in 96-well format.High Speed: The entire process only takes about half an hour.West Nile virus (WNV) is from the family Flaviviridae3 found in both tropical andtemperate regions. The main route of human infection is through the bite of an infected mosquito Infection causes severe neurological disease and fatalities in both human and animal hosts. There is currently no effective vaccine or antiviral drug to protect against WNV infection.4WNV contains a single-stranded, positive-sense RNA genome, which encodes threestructural proteins (capsid (C), membrane (M), envelope (E)), and seven non-structural proteins(NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5).5, 6 The West Nile viral NS3 protease is absolutelyessential (along with viral-encoded cofactor NS2B) for post-translational processing of a viralpolypeptide precursor in infected host cells. This polypeptide provides the structural and functional viral proteins, and inhibition of its processing could represent a potential treatment for viral infections. Thus, this protease has become one of the key targets for developing anti-WNV drugs.1,7The 440 West Nile Virus Protease Assay Kit provides a convenient, homogeneous assay for high throughput screening of West Nile Virus protease NS3 inhibitors. It allows for continuous quantification of protease activity using a fluorogenic peptide described in the literature.1 This peptide, Pyr-RTKR-AMC generates the AMC (7-amino-4-methylcoumarin) fluorophore upon NS3 protease cleavage. AMC has bright blue fluorescence that can be detected with excitation at 354nm and emission at 442nm.Kit Components:Component A Microtiter Plate 1x500 wells Pyr-RTKR-AMC, WNV protease substrate, Ex/Em=354 nm/442nm 5mM DMSO solution, 1x250ulComponent B AMC, fluorescence reference standard, Ex/Em=354nm/442nm5 mM DMSO solution, 1x10ulComponent C Assay Buffer 2 x 50mlComponent D WNV Protease Inhibitor undeca-D-Arg-NH2 1 mM DMSO solution, 1x10ulMaterials Required (but not provided):• WNV protease: WNV protease can be produced in an E. coli expression system.1, 2 (active recombinant WNV protease available)• 96-Well microplate: Black microplates can provide better signal to noise ratio.• Fluorescence microplate reader: Capable of detecting emission at 442nm with excitation at 354nm.Storage and Handling:• Store all kit components at -20°C.• Protect Components A and B from light and from moisture.• Component C can be stored at room temperature for convenience.
- 产品特点
- 针对West Nile Virus Protease, Fluorometric 440, BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到West Nile Virus Protease, Fluorometric 440, BioAssay Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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