逆转录病毒载体可用于将感兴趣的基因递送到期望整合到基因组中的靶细胞中。然而,传统的逆转录病毒表达技术导致病毒滴度较低,使得基因表达研究面临挑战。铂系列逆转录病毒表达系统结合了优质的包装细胞系和载体技术,通过单质粒转染即可产生高滴度的病毒。该系统包括我们独有的铂泛嗜性(Platinum-GP)包装细胞系,其包含病毒包装所需的gag和pol基因。只需将您感兴趣的基因克隆到提供的载体中,并与提供的水疱性口炎病毒糖蛋白(VSVG)质粒一起共转染到包装细胞中即可。 Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-GP Packaging cell line which contains the gag and pol genes necessary for viral packaging. Simply clone your gene of interest into the vector provided and co-transfect into the packaging cells along with the included VSVG plasmid.