Rpt2 is an AAA-ATPase subunit situated in the base sub-complex of the 19S proteasome regulator responsible for protein unfolding and stabilising the interaction of the 19S regulator with the 20S proteasome core. Recombinant protein expressed with N-terminal His-tag
Background:
Proteasomes are non-lysosomal proteolytic complexes localised primarily in the cytoplasm and in the nucleus of eukaryotic cells. The 26S proteasome structure is composed of a 20S proteasome catalytic core complex and one or two 19S (PA700) regulatory subcomplexes (26S and 30S complexes respectively). The 20S core comprises two copies of 14 subunits (7 alpha subunits and 7 beta subunits) arranged in a
