- 中文名称
Vitamin H, BioAssay ELISA Kit (Biotin, Coenzyme R, Bioepiderm)
- 英文名字
- Vitamin H, BioAssay ELISA Kit (Biotin, Coenzyme R, Bioepiderm)
- 供应商
- USBiological
- 产品货号
- V2143-80
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Biotin (Vitamin H) serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysine residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide. The awareness of the population for good health and its interest in healthy nutrition has increased significantly during the last years. After the content of vitamins in his nourishment has gained importance for the consumer, food has partially been vitaminized by the manufacturer. When there exists a lack of biotin, seborrhoea, dermatitis, anorexia, muscle pain, tiredness and nervous disorders can appear. Where biotin is synthesized by the human intestinal flora, deficiency symptoms are rare. Deficiency symptoms appear during excessive ingestion of raw egg white, which can be explained by its content of biotin-binding avidin.Principle of the Test:This Biotin (Vitamin H) quantitative test is based on the principle of enzyme linked immunosorbent assay. Avidin, which shows a high affinity to biotin, is bound on the surface of a microtiter plate. Biotin containing samples or standards and a biotin-alkaline phosphatase conjugate are added into the wells of the microtiter plate. Enzyme-labeled and free biotin compete for the binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 30 minutes, resulting in the development of a yellow color. The color development is inhibited by the addition of a stop solution. The yellow color is measured photometrically at 405nm. The concentration of biotin is indirectly proportional to the color intensity of the test sample.Intended Use:Vitamin H, BioAssay ELISA Kit (Biotin, Coenzyme R, Bioepiderm) is designed for the quantitative determination of Biotin (Vitamin H) in food.Sensitivity: 0.5ng/mlRecovery: 98%Incubation Time: 90 minutesKit Components:V2143-80A: Microtiter Strips, 1x96 wellls V2143-80B: Standard 0ng/ml, 1x500ul V2143-80C: Standard 1ng/ml, 1x500ul V2143-80D: Standard 2.5ng/ml, 1x500ul V2143-80E: Standard 5ng/ml, 1x500ul V2143-80F: Standard 10ng/ml, 1x500ul V2143-80G: Standard 25ng/ml, 1x500ul V2143-80H: Biotin-AP Conjugate (Biotin-Alkaline Phophatase), 1x15ml V2143-80J: Substrate Solution, 1x15ml V2143-80K: Stop Solution, 1x15ml V2143-80L: Standard/Sample Diluent, 2x50mlV2143-80M: Wash Solution (10X), 1x30mlStorage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Vitamin H, BioAssay ELISA Kit (Biotin, Coenzyme R, Bioepiderm)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vitamin H, BioAssay ELISA Kit (Biotin, Coenzyme R, Bioepiderm)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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