- 中文名称
Vitamin D Binding Protein, Human, BioAssay ELISA Kit (DBP, VDB, Gc-globulin, Group-specific Component, GC)
- 英文名字
- Vitamin D Binding Protein, Human, BioAssay ELISA Kit (DBP, VDB, Gc-globulin, Group-specific Component, GC)
- 供应商
- USBiological
- 产品货号
- V2130-10M
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Vitamin D-binding protein (DBP, VDBP), also called group-specific component (Gc) and macrophage-activating factor (GcMAF/DBP-MAF), is 52 to 58kD plasma glycoprotein with many functions, such as transport of vitamin D metabolites, control of bone development, binding of fatty acids, sequestration of actin, and modulating immune and inflammatory responses. DBP is synthesized predominantly by hepatic parenchymal cells and detected in plasma, cerebrospinal fluid, seminal fluid, saliva and breast milk. The exploitation of the unique properties of DBP could enable the development of important therapeutic agents such as vitamin D-associated conditions, actin sequestering in trauma and inflammation, chronic obstructive pulmonary disease, osteopetrosis, cancer therapy and immune modulation by macrophage activation. The DBP molecule is therefore an ideal candidate molecule for further investigation by biotechnology-based companies seeking a platform from which to pursue new therapeutic options.Intended Use:Human VDBP ELISA kit is to be used for the in vitro quantitative determination of human VDBP in human serum, human plasma, cell lysate and buffered solution. The assay will recognize native human VDBP. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity:The minimal detectable dose of human VDBP was calculated to be 2.8ng/ml, by subtracting two standard deviations from the mean of 12 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no cross-reactivity: human serum albumin, transferrin, IgG, alpha-fetoprotein(AFP), fibrinogen, plasminogen, Hemoglobin.Test Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human Fibrinogen. Samples are pippetted into these wells. Nonbound fibrinogen and other components of the sample should be removed by washing, then biotin- conjugated monoclonal antibody specific to fibrinogen added. In order to quantitatively determine the amount of fibrinogen present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured VDBP.Kit Components:V2130-10M1: Microplate: 1x96 wellsV2130-10M2: Incubation Buffer: 1x30ml, contains 0.05% Tween 20V2130-10M3: Washing Buffer, (20x): 2x25ml, contains 0.5% Tween 20V2130-10M4: Standard Protein (lyophilized): 1x 1vial, contains 0.1% sodium azideV2130-10M5: Standard/Sample Dilution Buffer: 1x25mlV2130-10M6: Second Antibody (lyophilized): 1x 1vial V2130-10M7: AV-HRP, (100x): 1x150ulV2130-10M8: Secondary Antibody/AV-HRP Dilution Buffer: 1x25ml, contains 0.1% sodium azideV2130-10M9: Substrate (TMB): 1x15ml, ≤ 0.05% TMBV2130-10M10: Stop Solution: 1x15ml, 1N sulfuric acidStorage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Vitamin D Binding Protein, Human, BioAssay ELISA Kit (DBP, VDB, Gc-globulin, Group-specific Component, GC)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vitamin D Binding Protein, Human, BioAssay ELISA Kit (DBP, VDB, Gc-globulin, Group-specific Component, GC)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
