- 中文名称
Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Food)
- 英文名字
- Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Food)
- 供应商
- USBiological
- 产品货号
- V2126-01
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Vitamin B12 as a trace element belongs to the biologically important chelate formers. The basic unit consists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide and a dimethylbenzimidazol group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the absence of intrinsic factor. A pernicious anemia can be treated by a high dosage of vitamin B12.The existing detection procedures are mainly microbiological methods, but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation. With the present test kit it is possible, to determine vitamin B12 quantitatively in vitaminated food in a significantly faster way (2.5 to 4 hours inclusive sample pretreatment) compared with a conventional microbiological assay (24 to 48 hours).Intended Use:For the quantitative determination of Vitamin B12 in food.Sensitivity: 0.3ng/mlRecovery (spiked samples): ~98%Incubation Time: 80 minTest Principle:The United States Biological Vitamin B12 quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against vitamin B12 is bound on the surface of a microtiter plate. Vitamin B12 containing samples or standards and a vitamin B12-peroxidase conjugate are given into the wells of the microtiter plate. Enzyme labeled and free vitamin B12 compete for the antibody binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450nm. The concentration of vitamin B12 is indirectly proportional to the color intensity of the test sample.Kit Components:V2126-01A: Microtiter Plate, 1x96 wells each, coated with anti-vitamin B12.V2126-01B: Standard 0ng/ml, 1x500ul, ready to use.V2126-01C: Standard 0.4ng/ml, 1x500ul, ready to use.V2126-01D: Standard 1ng/ml, 1x500ul, ready to use.V2126-01E: Standard 4ng/ml, 1x500ul, ready to use.V2126-01F: Standard 10ng/ml, 1x500ul, ready to use.V2126-01G: Standard 40ng/ml, 1x500ul, ready to use.V2126-01H: Vitamin B12 (HRP), 1x6ml ,ready-to-use.V2126-01J: Substrate Solution (TMB), 1x15ml, ready to use.V2126-01K: Stop Solution (0.5M H2SO4), 1x15ml, ready to use.V2126-01L: Sample Diluent, 2x60ml, ready to use.V2126-01M: Washing Solution, 10x, 1x30ml Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Sensitivity: 0.3ng/mlRecovery (spiked samples): ~98%Incubation Time: 80 minIntra-assay Precision: 3%
- 产品特点
- 针对Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Food)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vitamin B12 (VB12, Cyanocobalamin) BioAssay ELISA Kit (Food)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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