- 中文名称
Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12) BioAssay ELISA Kit (Human)
- 英文名字
- Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12) BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- V2118-14
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- 产品描述
- Human Vaspin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of vaspin in human serum and heparin plasma.Test Principle:Human Vaspin ELISA ELISA, standards, quality controls and samples are incubated in microtitration wells pre-coated with polyclonal anti-human vaspin antibody. After a 60 minute incubation followed by washing, biotin labelled polyclonal anti-human vaspin antibody is added and incubated with the captured vaspin for 60 minutes. After another washing, streptavidin- HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of vaspin. A standard curve is constructed by plotting absorbance values against vaspin concentrations of standards, and concentrations of unknown samples are determined using this standard curve.Features:• It is intended for research use only • The total assay time is less than 3.5 hours • The kit measures vaspin in serum and heparin plasma • Assay format is 96 wells • Standard is recombinant protein based • Quality Controls are human serum based • Components of the kit are provided ready to use, concentrated or lyophilizedPerformance Characteristics:• Sensitivity: Limit of Detection (LOD) (defined as concentration of vaspin giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: Ablank + 3xSDblank) is calculated from the real human vaspin values in wells and is 0.01ng/ml. *Dilution Buffer is pipetted into Blank wells.Range:0.031-2ng/ml Sample Volume:100ul/wellKit Components:V2118-14A: Microtiter Strips, 1x96 wells V2118-14B: Standard, 2x1vialV2118-14C: High Control, 0.79-1.19ng/ml, 2x1vialV2118-14D: Low Control, 0..12-0.18ng/ml, 2x1vialV2118-14E: Pab (Biotin) 100X, 1x130ulV2118-14F: Streptavidin (HRP), 1x13mlV2118-14G: Biotin Diluent, 1x13mlV2118-14H: Dilution Buffer, 1x20mlV2118-14J: Wash Solution (10X), 1x100mlV2118-14K: TMB, 1x13mlV2118-914L: Stop Solution, 1x13mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12) BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12) BioAssay ELISA Kit (Human)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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