- 中文名称
Vascular Endothelial Growth Factor Receptor 2 ELISA Kit, BioAssay (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)
- 英文名字
- Vascular Endothelial Growth Factor Receptor 2 ELISA Kit, BioAssay (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)
- 供应商
- USBiological
- 产品货号
- V2110-15M
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Total VEGFR-2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total VEGFR-2 protein. A VEGFR-2 Mouse Monoclonal Antibody has been coated onto the microwells. After incubation with cell lysates, both nonphospho-and phospho-VEGFR-2 proteins are captured by the coated antibody. Following extensive washing, a VEGFR-2 Rabbit Monoclonal Antibody is added to detect the captured VEGFR-2 protein. HRP-linked anti-rabbit antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total VEGFR-2 protein.Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding, and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCr, as well as Shb (5, 6). The signaling of VEGFR-2 is necessary for the execution of VEGFstimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (4).Kit Components:VEGFR-2 Mouse Monoclonal Antibody coated Microtiter plate, 1x96 wellsVEGFR-2 Detection Antibody, 1x11mlAnti-rabbit IgG labled with HRP, 1x11mlTMB Substrate, 1x11mlSTOP Solution, 1x11mlWash Buffer (20X), 1x25mlSample Diluent, 1x 25mlCell Lysis Buffer (10X), 1x15mlStorage and Stability:Kit should be stored at 4°C wtih the exception of 10X Cell Lysis Buffer, which is stored at -20°C Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Vascular Endothelial Growth Factor Receptor 2 ELISA Kit, BioAssay (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Vascular Endothelial Growth Factor Receptor 2 ELISA Kit, BioAssay (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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