- 中文名称
Uric Acid Assay Kit, BioAssay
- 英文名字
- Uric Acid Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- U2015-06V
- 产品报价
- ￥1/250Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Uric acid is the waste product produced from the degradation of purines. In healthy human, uric acid is filtered and removed from the blood by the kidneys and excreted into urine. Because a number of kidney diseases are known to affect uric acid levels, uric acid determination is thus important and useful in diagnosing and evaluating kidney diseases. For example, when uric acid is present in the blood at abnormally high levels, it tends to crystallize in body joints, resulting in gout, a very painful inflammatory condition. Increased levels of uric acid are also known to be associated with uremia, leukemia and pneumonia.Simple, direct and automation-ready procedures for measuring uric acid concentration in blood are becoming popular in Research and Drug Discovery. Uric acid assay kit is designed to measure uric acid directly in serum without any pretreatment. The improved method utilizes 2,4,6-tripyridyl-s-triazine that forms a blue colored complex specifically with iron in the presence of uric acid. The intensity of the color, measured at 590nm, is directly proportional to the uric acid concentration in the serum. The optimized formulation substantially reduces interference by substances in the raw samples.Key Features:Sensitive and accurate. Use 5ul samples. Linear detection range 0.22mg/dL (13uM) to 30 mg/dL (2380uM) uric acid in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.Applications:Direct Assays: uric acid in serum, plasma, urine and other biological samples.Drug Discovery/Pharmacology: effects of drugs on uric acid metabolism.Kit Contents: (250 tests in 96-well plates)U2015-06V1: Reagent A, 2x25mlU2015-06V2: Reagent B, 1x6mlU2015-06V3: Reagent C, 1x6mlU2015-06V4: Standard 10mg/dL uric acid, 1x1ml U2015-06V5: Blank Control, 1x1mlStorage conditions. The kit is shipped at room temperature. Store reagents at 4 °C, standard and blank control at -20 °C. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Reagent Preparation: shake Reagent C before use. Prepare enough working reagent by mixing 10 volumes of Reagent A, 1 volume Reagent B and 1 volume Reagent C. Fresh reconstitution is recommended. Equilibrate to room temperature before assay. Metal chelators (e.g. EDTA) interfere with this assay and should be avoided.Procedure using 96-well plate:1. Set up standards and samples. Transfer 5ul Blank, Standard and samples in duplicate wells of a clear bottom 96-well plate.2. Add 200ul working reagent and tap lightly to mix.3. Incubate 30 min at room temperature and read optical density at 510-630nm (peak absorbance at 590nm).Procedure using cuvette:1. Set up test tubes labeled Blank, Standard, Samples. Transfer 20ul Blank, Standard and samples to appropriately labeled tubes.2. Add 1000ul working reagent and tap lightly to mix.3. Incubate 30 min at room temperature and read optical density at 590nm (510nm-630nm).Calculation:The uric acid concentration of Sample is calculated as= X 10 (mg/dL)ODSAMPLE–ODBLANKODSTANDARD–ODBLANKODBLANK, ODSTANDARD and ODSAMPLE are OD590nm values of Blank, Standard and Sample, respectively. It is not necessary to prepare a calibration curve, because the concentration of the provided standard lies within the linear range.Normal serum uric acid values: 1.0 to 7.0 mg/dL.Conversions: 1 mg/dL uric acid equals 59.5uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipeting devices and accessories (e.g. 5ul).Procedure using 96-well plate:Clear bottom 96-well plates (e.g. Corning Costar).96-well plate absorbance (590nm) reader.Procedure using cuvette:Cuvets for measuring optical density at 510-630nm.Spectrophotometer for measuring absorbance at 590nm.Examples:Samples were assayed using the 96-well protocol. The uric acid content (mg/dL) was 1.3 ± 0.1 (n=4) for mice serum, 2.6 ± 0.0 (n=4) for fetal bovine serum (Invitrogen), 1.4 ± 0.1 for goat serum, 1.3 ± 0.1 for rat serum, 2.9 ± 0.1 for rat plasma, 3.4 ± 0.1 for human serum and 1.4 ± 0.1 for human plasma, respectively.
- 产品特点
- 针对Uric Acid Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Uric Acid Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
