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细胞分析试剂盒
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中文名称

Urea Assay Kit, BioAssay

英文名字
Urea Assay Kit, BioAssay
供应商
USBiological
产品货号
U2010-03
产品报价
¥1/500Tests
产品说明书
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背景资料
产品描述
Urea is primarily produced in the liver and secreted by the kidneys. Urea is the major end product of protein catabolism in animals. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases, e.g., congestive heart failure, liver diseases and diabetes. Decreased levels indicate acute hepatic insufficiency or may result from over-vigorous parenteral fluid therapy. Simple, direct and automation-ready procedures for measuring urea concentration or blood urea nitrogen BUN in biological samples are becoming popular in Research and Drug Discovery. Urea assay kit is designed to measure urea directly in biological samples without any pretreatment. The improved Jung method utilizes a chromogenic reagent that forms a colored complex specifically with urea. The intensity of the color, measured at 520nm, is directly proportional to the urea concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples. Key Features:Sensitive and Accurate: Use 5ul of samples. Linear detection range 0.08mg/dl (13mM) to 100mg/dl (17mM) urea in 96-well plate assay. Simple and High-throughput: The procedure involves addition of a single working reagent and incubation for 20 minutes. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved Reagent Stability and Versatility: The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low Interference in Biological Samples: No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.Applications:Direct Assays: Urea in serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL) etc. Drug Discovery/Pharmacology: Effects of drugs on urea metabolism. Environment: urea determination in waste water, soil etc. Kit Components: U2010-03A: Reagent A, 1x50ml U2010-03B: Reagent B, 1x50ml *U2010-03C: Standard (50mg/dL), 1x1mlStorage and Stability:Store *U2010-03C at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Urea Assay Kit, BioAssay该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Urea Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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