- 中文名称
Tumor Necrosis Factor beta, Human (TNFb) BioAssay ELISA Kit
- 英文名字
- Tumor Necrosis Factor beta, Human (TNFb) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T9160-40A
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- For the quantitative determination of human Tumor Necrosis Factor-b (TNF-b) concentrations in serum, plasma, cell culture supernatant, and other biological fluids.This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-b present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody-enzyme specific for TNF-b is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-b bound in the initial step. The color development is stopped and the intensity of the color is measured.In order to measure the concentration of TNF-b in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TNF-b concentration (pg/ml). The concentration of TNF-b in the samples is then determined by comparing the O.D. of the samples to the standard curve. This immunoassay is calibrated against natural human TNF-b (NIBSC/WHO First International Standard 87/640).Kit Components:T9160-40C: TNFb Microtiter Plate, 1x96wells (Pre-coated with a monoclonal anti-human TNFb)T9160-40D: Pab (Biotin) Conjugate, 1x11ml (Anti-human TNFb labeled with Biotin)T9160-40E: Avidin (HRP) Conjugate, 1x14mlT9160-40F: TNFb Standard 0.8ng/vial, 2x1vialT9160-40G: Serum Assay Buffer, 1x6mlT9160-40H: Calibrator Diluent I, 1x22mlT9160-40J: Calibrator Diluent II, 1x22mlT9160-40K: Wash Buffer, 20X, 1x60mlT9160-40L: Substrate A, 1x10mlT9160-40M: Substrate B, 1x10mlT9160-4N: Stop Solution, 1x14mlSensitivity: Calibrator Diluent I: 22pg/mlCalibrator Diluent II: 11pg/mlAssay Range: 125-8000pg/mlStorage and Stability: Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Tumor Necrosis Factor beta, Human (TNFb) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Tumor Necrosis Factor beta, Human (TNFb) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
