- 中文名称
Tumor Necrosis Factor alpha, Human (TNFa) BioAssay ELISA Kit
- 英文名字
- Tumor Necrosis Factor alpha, Human (TNFa) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T9160-01
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Tumor Necrosis Factor alpha (TNF-a), also known as cachectin was initially named for its remarkable ability to cause hemorrhagic necrosis of tumors in mice. The TNF-a gene located on chromosome 6 encodes a 233aa prohormone bound to the plasma membrane, with the mature form (157aa, 17kD) exposed on the extracellular surface. Soluable, mature TNF-a is released upon cleavage of the C-terminal. The primary source in vivo of TNF-a is thought to be the monocyte/macrophage but various cell types are known to express this cytokine such as lymphocytes, basophils, eosinophils, mast cells, NK cells, T cells, B cells, kerantinocytes, Kupffer cells, astrocytes, and some types of tumors. TNF-a is produced upon stimulation with cytokines such as IL-1, 1L-2, GM-CSF, TNF-a itself and with bacterial lipopolysaccharide (LPS) which is a potent inducer. TNF-a once produced and secreted will bind to TNF-a pleiotropic cytokine that can induce disease through TNF-a toxicity (tissue injury, catabolic illness, and mediating shock) and improve host defence mechanisms (stimulating inflammation and increasing immune cell function). In the future, therapies may be developed by blocking TNF-a harmful effects and enhancing TNF-a beneficial effects.This TNF-a enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to TNF-a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TNF-a and incubated. TNF-a if present, will bind and become immobilized by the antibody pre-coated on the wells and then be sandwiched by biotin conjugate. In order to measure the concentration of TNF-a in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TNF-a concentration (pg/ml). The concentration of TNF-a in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: Calibrator Diluent I: 2.4pg/mlCalibrator Diluent II: 2.4pg/mlRange:15.625-1000pg/mlKit Components:T9160-01A: TNFa Microtiter plate, 1x96 wells T9160-01B: Pab (Biotin) 1x 7ml T9160-01C: Avidin (HRP) 1x12ml T9160-01D: TNF-a Standard, 2x1vialT9160-01E: Calibrator Diluent I, 1x22mlT9160-01F: Calibrator Diluent II ,1x22ml T9160-01G: Wash Buffer, 20X 1x60ml T9160-01H: Substrate, A 1x11ml T9160-0J: Substrate B, 1x11ml1T9160-01K: Stop Solution, 1x14ml 2N Sulphuric Acid (H2SO4). Storage and Stability:Store kit components at 4°C except *T9160-01D. Stable for 6 months. Store T9160-01D lyophilized powder at 4°C. Store reconstituted stock at -20°C for <30 days. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- 产品特点
- 针对Tumor Necrosis Factor alpha, Human (TNFa) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Tumor Necrosis Factor alpha, Human (TNFa) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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