- 中文名称
Triiodothyronine, Free (fT3, T3) Human, BioAssay ELISA Kit
- 英文名字
- Triiodothyronine, Free (fT3, T3) Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- T8425-07F
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- 产品描述
- Sample Type:SerumIntended Use:For the quantitative determination of Free Triiodothyronine (fT3) concentration in human Serum.Introduction:L-Triiodothyronine, a thyroid hormone, circulates in blood almost completely bound (> 99.5%) to carrier proteins. The main transport protein is thyroxine-binding globulin (TBG). However, only the free (unbound) portion of triiodothyronine is believed to be responsible for the biological action. Furthermore, the concentrations of the carrier proteins are altered in many conditions, such as pregnancy. In individuals with normal thyroid function, as the concentrations of the carrier proteins change, the total T3 levels change in consert so that the free thriiodothyronine (fT3) concentration remains constant. Thus, measurements of fT3 concentrations correlate more reliably with clinical status than total triiodothyronine levels. For example, the increase in total triiodothyronine levels associated with pregnancy, oral contraceptives, and estrogen therapy result in higher total T3 levels while the fT3 concentration remains basically unchanged. This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations in a direct determination of fT3.Test Principle:The fT3 test is a solid phase competitive enzyme immunoassay. Serum samples, standards, and T3-Enzyme Conjugate Working Reagent is added to wells coated with monoclonal T3 antibody. fT3 in the sample specimen and the T3 labeled conjugate compete for available binding sites on the antibody. After a 60 minutes incubation at room temperature, the wells are washed with water to remove unbound T3 conjugate. A solution of H2O2/TMB is then added and incubated for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of 3N HCl, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled fT3 in the sample. By reference to a series of fT3 standards assayed in the same way, the condentration of fT3 in the unknown sample is quantified.Kit Components:T3 Antibody-Coated Microplate, 96 wellsT3-Enzyme Conjugate, 10.5ml, ready to use.Standard (0pg/ml), 1x1mlStandard (0.9pg/ml), 1x1mlStandard (2.2pg/ml), 1x1mlStandard (5pg/ml), 1x1mlStandard (9pg/ml), 1x1mlStandard (19pg/ml), 1x1mlColor Reagent A, 13mlColor Reagent B, 13mlStop solution (3N HCI), 10mlStorage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Triiodothyronine, Free (fT3, T3) Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Triiodothyronine, Free (fT3, T3) Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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