- 中文名称
Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)
- 英文名字
- Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)
- 供应商
- USBiological
- 产品货号
- T8425-04
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- 产品描述
- Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3) permits the measurement of the total amount of binding sites available for the thyroid hormones in human serum or plasma by a microplate enzyme immunoassay.Triiodothyronine also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. Together with thyroxine (T4), T3 exerts a potent influence on the basal metabolic rate. T3 is synthesized from the tyrosine residues of thyroglobin and is found in the serum of normal individuals. Although T3 has a lower serum concentration than T4, it is 5 to 10 times more active.This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations. In this method, serum reference, patient specimen, or control is first added to a microplate well. Enzyme-T3 conjugate and thyroxine (T4) are added, and then the reactants are mixed. The endogenous binding proteins of the sample react with the thyroxine, but not with the enzyme conjugate. This leads to a higher binding of the enzyme conjugate to the antibody combining sites, reactive for triiodothyronine and thyroxine, immobilized on the well as the binding capacity of the specimen increases. After the completion of the required incubation period, the antibody bound enzyme-triiodothyronine conjugate is separated from the unbound enzyme-triiodothyronine conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color. The employment of several serum references of known unsaturated thyroid hormone binding capacity permits construction of a graph of absorbance and concentration. From comparison to the dose response curve, an unknown specimen's absorbance can be correlated with thyroid hormone binding capacity.Kit Components: T8425-04A: Microtiter Plate, 1x96 wellsT8425-04B: Calibrator, 18%U, 1x1mlT8425-04C:. Calibrator, 24.5%U, 1x1mlT8425-04D: Calibrator, 34%U, 1x1mlT8425-04E: Calibrator, 48%U, 1x1mlT8425-04F. T3 (HRP), 1x1.5mlT8425-04G: HRP Buffer, 1x13mlT8425-04H: Substrate A (TMB), 1x7mlT8425-04J: Substrate B (H2O2), 1x7mlT8425-04K: Wash Buffer, 50X, 1x20mlT8425-04L: Stop Solution (1N HCl), 1x8ml Storage and Stability:Store all components at 4°C. Stable for 6 months. Opened reagents are stable for 60 days at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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