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中文名称

Transforming Growth Factor beta1, Human (TGFb1) BioAssay ELISA Kit

英文名字
Transforming Growth Factor beta1, Human (TGFb1) BioAssay ELISA Kit
供应商
USBiological
产品货号
T8250-15B
产品报价
¥1/96Tests
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产品描述
This Transforming Growth Factor beta1, Human (TGFb1) BioAssay ELISA Kit is a 3 hour solid phase immunoassay readily applicable to measure TGFb1 levels in serum, plasma, cell culture supernatant and other biological fluids in the range of 0 to 2500pg/m. It showed no cross reactivity with various other cytokines superfamily proteins. This TGFb1 ELISA kit is expected to be effectively used for further investigations into the relationship between TGFb1 and various diseases.Transforming Growth Factor beta1, Human (TGFb1) BioGene ELISA Kit, is to be used for the in vitro quantitative determination of human transforming growth factor beta 1 (TGF-b1) concentrations in serum, plasma, cell culture supernatant and other biological fluids. It is intended for research use only and is not for use in diagnostic procedures.Sensitivity:Calibrator Diluent I: ≥9.0pg/mlCalibrator Diluent II: ≥9.0pg/mlRange: 78.1-2500pg/mlKit Components:8250-15B1: TGFb1 Microtiter Plate, 1x96 wells. Pre-coated with anti-human TGFb1 monoclonal antibody.T8250-15B2: TGFb1 (Biotin), 1x7ml. Anti-human TGFb1 polyclonal antibody conjugated to Biotin. T8250-15B3: Avidin (HRP), 1x14ml.Avidin conjugated to horseradish peroxidase.T8250-15B4: TGFb1 Standard, 2x1vials. Recombinant human TGFb1 (4000pg/vial) in a buffered protein base with preservative, lyophilized.T8250-15B5: Calibrator Diluent I, 1x25ml. Animal protein with buffer and preservative. For TGFb1 serum/plasma testing.T8250-15B6: Calibrator Diluent II, 1x25ml. Cell culture medium with animal protein and preservative. For TGFb1 cell culture supernatant testing.T8250-15B7: Wash Buffer (20X), 1x60ml. 20-fold concentrated solution of buffered surfactant.T8250-15B8: Substrate A, 1x10ml. Buffered solution with H2O2.T8250-15B9: Substrate B, 1x10ml. Buffered solution with TMB.T8250-15B10: Stop Solution, 1x14ml. 2N Sulphuric Acid (H2SO4).Specificity: Kit recognizes both natural and recombinant human TGF-b1. The factors listed below were prepared at 50pg/ml. In Calibrator Diluent I and Calibrator Diluent II and assayed for cross-reactivity. Preparations of the following factors at 50 pg/ml. In a mid-range hTGF-b1Control were assayed for interference. No significant crossreactivity or interference was observed.Crossreactivity:FactorConcentration Tested (pg/ml)Observed Value (pg/ml)% Cross-reactivityrhLatent TGF-b110,0001.24412.4rh TGF-b1,210,0006716.7rh TGF-b250,00041.90.08rh TGF-b350,00058.40.12rh TGF-b550,0009621.9Storage and Stabiity:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
产品特点
针对Transforming Growth Factor beta1, Human (TGFb1) BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Transforming Growth Factor beta1, Human (TGFb1) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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