- 中文名称
Transferrin Receptor, soluble, Human, BioAssay ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)
- 英文名字
- Transferrin Receptor, soluble, Human, BioAssay ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)
- 供应商
- USBiological
- 产品货号
- T8199-38
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- 产品描述
- Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease. Principle:Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody’sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.Specificity: Human soluble transferrin receptor Sample Volume: 100ul/well Range: 0.05-2ug sTfR /1ml of sample Sensitivity: 2ng/ml Assay Time: The total assay time is less than 3 hours (incubation at the temperature of 30°C is necessary). Kit Components:T8199-38A: Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab T8199-38B: Mab (HRP), 1x13mlT8199-38C: Standard, 2ug/ml, 1x100ul T8199-38D: Standard, 1ug/ml, 1x100ul T8199-38E: Standard, 0.5ug/ml, 1x.100ul T8199-38F: Standard, 0.2ug/ml, 1x100ul T8199-38G: Standard, 0.1ug/ml, 1x100ul T8199-38H: Standard, 0.05ug/ml, 1x100ulT8199-38J: Quality Control, High, 50X, (5.5-8.3ug /ml), 1x50ulT8199-38K: Quality Control, Low, 50X, (1.3-2.0ug/ml), 1x50ulT8199-38L: Dilution Buffer, 2x13mlT8199-38M: Wash Solution, 10X, 1x100mlT8199-38N: Substrate Solution (TMB), 1x13mlT8199-38P: Stop Solution (0.2M H2SO4), 1x13ml Storage and Stability:Store kit at 4°C. Unopened kit components are stable for 6 months after receipt. Opened reagents are stable for 3 months when stored at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Transferrin Receptor, soluble, Human, BioAssay ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Transferrin Receptor, soluble, Human, BioAssay ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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