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细胞分析试剂盒
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中文名称

Tissue, DNA, RNA, Protein, Extraction Kit, BioAssay

英文名字
Tissue, DNA, RNA, Protein, Extraction Kit, BioAssay
供应商
USBiological
产品货号
T5595-0004
产品报价
¥1/1Kit
产品说明书
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产品描述
Isolation from the same piece of tissue simultaneouslyDescriptionIn order to study the function of DNA, protein, RNA and their relationship, it is very important to get RNA, DNA and protein from the same piece of tissue. DNA, Protein, RNA Extraction Kit provides a convenient and efficiency system for isolation of RNA, DNA and protein from the same piece of tissue simultaneously. The isolated RNA can be used for mRNA isolation, probe generation, RT-PCR, Northern blot analysis, primer extension, RNA protection assay, and In vitro translation. The isolated DNA can be used for PCR amplification. And the isolated protein can be used for Western Analysis. The kit is enough for isolating DNA/RNA/Protein from 5 gram tissues or 10 gram cells or blood.Quality Control:A representative kit from the same lot is randomly selected for isolation of RNA, DNA and protein. The quality and purity of isolated total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. Beta-actin gene, 838 bp fragment, is successfully amplified from the isolated genomic DNA. And the isolated protein’s quality is ensured by Western blotting analysis with anti-GAPDH antibody.Kit Components:*If precipitate formed in solution 3, place the bottle at 65°C water bath to dissolve it before use.ItemAmountStoragePart No.1. Solution 150ml4°C2. Solution 26mlRT3. Solution 350mlRT*4. Phenol B50ml4°C5. 0.5% SDS25mlRT6. DEPC H2O/0.1mM EDTA50mlRT7. TE buffer5mlRT8. RNAase (10ug/ul)14ul-20°C
产品特点
针对Tissue, DNA, RNA, Protein, Extraction Kit, BioAssay该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Tissue, DNA, RNA, Protein, Extraction Kit, BioAssay产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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